Genetic-based approaches-including mutant screens population, microarray analysis, cloning and transgenesis have expanded our knowledge of gene function during meiosis in plants. Nonetheless, genetic tools are not without limitations. One alternative approach to studying meiosis plants, especially in polyploidy as Triticum aestivum L. (wheat bread), is proteomics. However, the proteins using proteomics-based approach is rarely depicted, with only two early learning efforts reported plant meiosis. Here, we report the initial investigation meiosis wheat bread using proteomics.
Five places differently expressed proteins were identified using 2D gel electrophoresis (2DGE) in the protein extracts of the four stages of meiosis were collected and three genotypes (wild-type Chinese Spring, ph1b and ph2a wheat mutant lines). mass spectrometry (MS / MS) identification tandem peptides from this protein led to the isolation and characterization of full-length clones of wheat Speckle-type POZ protein, a SF21-like protein and HSP70, and partial coding sequence of a hexose transporter.
Significantly, the function is suspected of Speckle-type POZ protein and HSP70 was confirmed using in vitro DNA binding test. Through the use of 2DGE proteomics approach, we show that proteomics is an alternative to the genetic-based approach when studying meiosis in wheat. More importantly, we reported a potential role for Speckle-type POZ protein and HSP70 in a chromosome pair during the early stages of meiosis in bread wheat.
Improved quantification of therapeutic proteins covalently aggregate by non-reducing capillary gel electrophoresis using sodium sulphate hexadecyl (CE-SHS)
Capillary gel electrophoresis (CGE) using sodium dodecyl sulphate (SDS-CGE or CE-SDS) is commonly used in the biotechnology industry to assess the purity of the complex therapy during the manufacturing process optimization and also for commercial release and stability testing. However, for therapeutic protein mAb-1 and mAb-2, non-reducing (NR) CE-SDS resulted in higher than expected% aggregates were much lower the purity obviously relative to the purity reported by complementary methods, such as Size Exclusion Chromatography (SEC ). In addition, a strong dependence of the protein load at the aggregate level was observed which prevents any reasonable assessment of the value of true purity.
The solution is to supplement the relatively hydrophobic SDS detergent hexadecyl sodium sulfate (SHS) in buffer gel sieving matrix protein that virtually eliminates the burden of dependency and reduce the value of the aggregate% to the level expected when compared to the SEC. Analytical ultracentrifugation (AUC) was used to help confirm the accuracy of the results of the SEC. This work underscores how to use detergents in addition to SDS in CGE applications can be valuable in commercial biological space and give examples of how the SEC can be used to confirm the accuracy of the data CGE.
Production of high-quality two-dimensional gel electrophoresis profiles of samples of marine medaka using Trizol-based protein extraction approaches.
Medaka Sea is one of the most popular models of fish species for Ecotoxicology and environmental research and study of proteomics is a useful tool for understanding the medaka molecular response after exposure to different environmental stresses. High-quality protein sample preparation is the key to producing high-quality two-dimensional gel electrophoresis (2-DE) results for proteomics analysis. In recent years, the extraction of protein-based Trizol has gained popularity because of its performance promise in producing high-quality 2-DE and comfort method.
Three Trizol-based approach (Trizol method, the method and the method of Trizol Trizol aliquots with commercial cleaning kit) is used to extract protein from marine samples medaka and 2-DE profiles produced. 2-DE profile quality and effectiveness of extraction methods were evaluated. For comparison, two common protein extraction method (method of lysis buffer and trichloroacetic acid (TCA) / acetone precipitation extraction) are also applied in parallel to Trizol based approaches.Any three Trizol-based approach produces high-quality 2-DE profiles of marine medaka compared with both methods of lysis buffer and TCA / acetone precipitation extraction.
ES 8/10 Double Gel Comb For ES Electrophoresis |
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LE1011-A3 | GenDepot | Ea | EUR 148.8 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-1 | Glycomatrix | 15 mL | EUR 17.3 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-2 | Glycomatrix | 25 mL | EUR 23.23 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-3 | Glycomatrix | 50 mL | EUR 35.42 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
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21440026-4 | Glycomatrix | 100 mL | EUR 62.99 |
CometAssay Single Cell Gel Electrophoresis Assay |
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4250-050-K | Biotechne | 50 tests | EUR 513.6 |
WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell |
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HTES1013 | Shanghai WSHT Biotechnology | set | EUR 602 |
WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell |
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HTES1014 | Shanghai WSHT Biotechnology | set | EUR 448 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
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E1WP3231 | EnoGene | 50T | EUR 100 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
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E1WP3232 | EnoGene | 100T | EUR 150 |
Gel Electrophoresis System with Time Control, 220-240 V |
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Q-EP-2114 | ABM | 1 | EUR 1180 |
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
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4251-050-K | Biotechne | 1 kit | EUR 684.96 |
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system) |
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E1WP324 | EnoGene | 50T | EUR 60 |
Gel Electrophoresis System with Muticaster, 100-120 V Input |
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Q-EP-2034 | ABM | 1 | EUR 1715 |
Gel Electrophoresis System with Muticaster, 220-240 V Input |
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Q-EP-2056 | ABM | 1 | EUR 2125 |
Starch (Smithies), for electrophoresis |
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GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 370.8 |
Starch (Smithies), for electrophoresis |
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GE6857-500G | Glentham Life Sciences | 500 g | EUR 217.2 |
Aminoacetic acid, ?For electrophoresis |
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GRM2233-100G | EWC Diagnostics | 1 unit | EUR 32.26 |
Description: Aminoacetic acid, ?For electrophoresis |
Aminoacetic acid, ?For electrophoresis |
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GRM2233-500G | EWC Diagnostics | 1 unit | EUR 144.96 |
Description: Aminoacetic acid, ?For electrophoresis |
Gel Electrophoresis System with Time Control, 100-120 V Input |
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Q-EP-2100 | ABM | 1 | EUR 1385 |
Acrylamide, suitable for electrophoresis |
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GK5651-100 | Glentham Life Sciences | 100 | EUR 23.8 |
Acrylamide, suitable for electrophoresis |
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GK5651-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Chiller for Electrophoresis systems - EACH |
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CSLCHILLER | Scientific Laboratory Supplies | EACH | EUR 7642.35 |
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system) |
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E1WP326 | EnoGene | 50T | EUR 100 |
Electrophoresis baffle for Life mini tank |
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HTDB03 | Shanghai WSHT Biotechnology | pcs | EUR 5.6 |
High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa) |
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MKPZ010-200mL | Shanghai WSHT Biotechnology | 200μL | EUR 72 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230 | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
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EV2230-US | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320 | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
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EV2320-US | Consort | ea | EUR 898.8 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150 | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150-UK | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
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EV3150-US | Consort | ea | EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330 | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
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EV3330-US | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620 | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
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EV3620-US | Consort | ea | EUR 1456.8 |
Electrophoresis Buffer (10×) |
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E-BC-R331-100mL | Elabscience Biotech | 100mL | EUR 15 |
Electrophoresis Buffer (10×) |
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E-BC-R331-200mL | Elabscience Biotech | 200mL | EUR 20 |
Electrophoresis Buffer (10×) |
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E-BC-R331-500mL | Elabscience Biotech | 500mL | EUR 25 |
Electrophoresis Buffer (10×) |
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E-BC-R331-each | Elabscience Biotech | each | Ask for price |
Electrophoresis baffle for Life Technology Novex |
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HTDB02 | Shanghai WSHT Biotechnology | pcs | EUR 2.8 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450 | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450-UK | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
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EV1450-US | Consort | ea | EUR 574.8 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310 | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
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EV2310-US | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650 | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
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EV2650-US | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020 | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020-UK | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
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EV3020-US | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610 | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610-UK | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
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EV3610-US | Consort | ea | EUR 960 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
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20840057-1 | Glycomatrix | 500 mL | EUR 74.66 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
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20840057-2 | Glycomatrix | 1 L | EUR 139.51 |
TEMED, electrophoresis grade |
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RM10358-100G | EWC Diagnostics | 1 unit | EUR 301.18 |
Description: TEMED, electrophoresis grade |
electrophoresis power supply |
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EV245 | Consort | ea | EUR 574.8 |
Electrophoresis Power Supply |
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LA690-1NO | EWC Diagnostics | 1 unit | EUR 432.84 |
Description: Electrophoresis Power Supply |
Electrophoresis baffle for Bio-Rad Mini-PROTEAN |
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HTDB01 | Shanghai WSHT Biotechnology | pcs | EUR 2.1 |
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
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ACT-CBMN-7 | ACTGene | each | EUR 892.8 |
10X MOPS Electrophoresis Buffer |
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ML050-500ML | EWC Diagnostics | 1 unit | EUR 32.7 |
Description: 10X MOPS Electrophoresis Buffer |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster) |
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HTES2001 | Shanghai WSHT Biotechnology | set | EUR 322 |
5 x SDS Electrophoresis Buffer |
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GR103039 | Genorise Scientific | 1 L | EUR 107 |
CometAssay Electrophoresis System |
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4250-050-ES | Biotechne | 1 Kit | EUR 3540.96 |
myGel Mini Electrophoresis System |
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E1101 | Benchmark Scientific | 1 each | EUR 636.38 |
myGel Mini Electrophoresis System |
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BM0286 | GenDepot | System | EUR 741.6 |
ES Horizontal Electrophoresis Set |
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LE1011 | GenDepot | Set | EUR 578.4 |
6X6 well Electrophoresis Apparatus |
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EDK626 | Bio Basic | 1 UNIT | EUR 750.6 |
miniONE Electrophoresis System - EACH |
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ELE3400 | Scientific Laboratory Supplies | EACH | EUR 777.6 |
electrophoresis power supply 120 vac |
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EV245-US | Consort | ea | EUR 574.8 |
electrophoresis power supply uk plug |
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EV245-UK | Consort | ea | EUR 574.8 |
ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels |
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LE1001 | GenDepot | Set | EUR 651.6 |
CometAssay Electrophoresis Starter Kit |
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4250-050-ESK | Biotechne | 1 Kit | EUR 3749.28 |
ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels |
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LE1002 | GenDepot | Set | EUR 699.6 |
ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels |
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LE1003 | GenDepot | Set | EUR 586.8 |
ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels |
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LE1004 | GenDepot | Set | EUR 886.8 |
ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels |
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LE1005 | GenDepot | Set | EUR 1081.2 |
ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels |
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LE1006 | GenDepot | Set | EUR 1170 |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP001-112RUNS | EWC Diagnostics | 1 unit | EUR 118.87 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP001-224RUNS | EWC Diagnostics | 1 unit | EUR 214 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
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MBP008-224RUNS | EWC Diagnostics | 1 unit | EUR 208.42 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Paper Electrophoresis Kit (Cell |
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MBP009-20RUNS | EWC Diagnostics | 1 unit | EUR 59.3 |
Description: Alkaline Paper Electrophoresis Kit (Cell |
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
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LA1070-1NO | EWC Diagnostics | 1 unit | EUR 1258.71 |
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
Electrophoresis Power Supply (2 termina |
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LA842-1NO | EWC Diagnostics | 1 unit | EUR 66.05 |
Description: Electrophoresis Power Supply (2 termina |
Cellulose Acetate Electrophoresis System |
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LA1027-1NO | EWC Diagnostics | 1 unit | EUR 534.37 |
Description: Cellulose Acetate Electrophoresis System |
WSHT-Basic Electrophoresis Power Supply |
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HTES1001 | Shanghai WSHT Biotechnology | set | EUR 557.2 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
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LE1011-A2 | GenDepot | Ea | EUR 181.2 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-10 | Glentham Life Sciences | 10 | EUR 28.4 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-100 | Glentham Life Sciences | 100 | EUR 142.4 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-25 | Glentham Life Sciences | 25 | EUR 50.6 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-250 | Glentham Life Sciences | 250 | EUR 284.8 |
Bovine Serum Albumin, 98% by electrophoresis |
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GE3699-500 | Glentham Life Sciences | 500 | EUR 506.2 |
4 x Phosphate/SDS Electrophoresis Buffer |
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GR103033 | Genorise Scientific | 1 L | EUR 116 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
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LE1011-A1 | GenDepot | Ea | EUR 198 |
Hi-Eco Mini Horizontal Electrophoresis K |
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LA851-1NO | EWC Diagnostics | 1 unit | EUR 205.56 |
Description: Hi-Eco Mini Horizontal Electrophoresis K |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
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EDK611 | Bio Basic | 1 UNIT | EUR 383.11 |
Electrophoresis Apparatus - 7x14cm (6-10-well) |
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EDK613 | Bio Basic | 1 UNIT | EUR 487.51 |
WSHT-Universal Electrophoresis Power Supply |
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HTES1003 | Shanghai WSHT Biotechnology | set | EUR 784 |
ComboMiniTM Horizontal Electrophoresis System (110V) |
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CBMN-7 | Avans Biotechnology Corp | each | EUR 500 |
ComboMiniTM Horizontal Electrophoresis System (220V) |
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CBMN-8 | Avans Biotechnology Corp | each | EUR 530 |
WSHT-miniPRO4 Mini Vertical Electrophoresis Cell |
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HTES1009 | Shanghai WSHT Biotechnology | set | EUR 795.2 |
WSHT-miniPRO2 Mini Vertical Electrophoresis Cell |
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HTES1010 | Shanghai WSHT Biotechnology | set | EUR 516.6 |
WSHT-easyPRO4 Mini Vertical Electrophoresis Cell |
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HTES1011 | Shanghai WSHT Biotechnology | set | EUR 837.2 |
WSHT-easyPRO2 Mini Vertical Electrophoresis Cell |
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HTES1012 | Shanghai WSHT Biotechnology | set | EUR 544.6 |
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
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EDK614 | Bio Basic | 1 UNIT | EUR 520.92 |
WSHT-miniDNA Mini Horizontal Electrophoresis Cell |
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HTES1008 | Shanghai WSHT Biotechnology | set | EUR 193.2 |
10-well protein Vertical Electrophoresis Apparatus |
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EDK692 | Bio Basic | 1 UNIT | EUR 487.51 |
Agar, High Gel Strength |
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40100064-1 | Glycomatrix | 500 g | EUR 84.23 |
Agar, High Gel Strength |
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40100064-2 | Glycomatrix | 1 kg | EUR 135.61 |
Agar, High Gel Strength |
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40100064-3 | Glycomatrix | 2 kg | EUR 256.75 |
Agar, High Gel Strength |
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40100064-4 | Glycomatrix | 5 kg | EUR 557.75 |
Agar, high gel strength |
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GK9085-1 | Glentham Life Sciences | 1 | EUR 142.4 |
Agar, high gel strength |
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GK9085-100 | Glentham Life Sciences | 100 | EUR 23.8 |
Agar, high gel strength |
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GK9085-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Agar, high gel strength |
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GK9085-1KG | Glentham Life Sciences | 1 kg | EUR 208.8 |
Agar, high gel strength |
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GK9085-250 | Glentham Life Sciences | 250 | EUR 47.4 |
Agar, high gel strength |
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GK9085-250G | Glentham Life Sciences | 250 g | EUR 93.6 |
Agar, high gel strength |
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GK9085-500 | Glentham Life Sciences | 500 | EUR 81.5 |
Agar, high gel strength |
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GK9085-500G | Glentham Life Sciences | 500 g | EUR 134.4 |
Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH |
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ELE1111 | Scientific Laboratory Supplies | EACH | EUR 961.2 |
WSHT-Multi-Functional Electrophoresis Power Supply |
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HTES1004 | Shanghai WSHT Biotechnology | set | EUR 896 |
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade) |
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E37F007 | EnoGene | 100-μL | EUR 226.67 |
Description: Reagents-for-ssDNA-or-RNA-electrophoresis |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell |
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HTES2001-P | Shanghai WSHT Biotechnology | set | EUR 459.2 |
WSHT-liteDNA Universal Horizontal Electrophoresis Cell |
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HTES1007 | Shanghai WSHT Biotechnology | set | EUR 259 |
myGel Mini Electrophoresis System 230V (includes transformer) |
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E1101-E | Benchmark Scientific | 1 PC | EUR 665.55 |
myGel Mini Electrophoresis System 230V (includes transformer) |
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BM0291 | GenDepot | System | EUR 789.6 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
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4250-050-ESK-PS2 | Biotechne | 1 Kit | EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
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4250-050-ESK-PS3 | Biotechne | 1 Kit | EUR 4597.68 |
In addition, the Trizol method with clean-up kit produced the best commercial 2-DE profile in terms of the clarity of the background, the number of spots and resolution proteins.Trizol based approach offered a better choice than traditional protein extraction method for 2-DE analysis of medaka sea. A modified version of the method Trizol with commercial clean-up kit was shown to produce the best 2-DE profile.