Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

protein extraction for two-dimensional electrophoresis of network recalcitrant species is quite problematic and challenging because of low protein content and a high abundance of contaminants. Proteomics in Shorea robusta is almost impossible due to lack of preparation procedures suitable protein. To establish a protocol for extracting proteins effectively suitable for electrophoresis two-dimensional in Shorea robusta, four procedures (borate buffer / extraction trichloroacetic acid, organic solvents / precipitation trichloroacetic acid, sucrose / Tris / phenol, and an organic solvent / phenol / sodium dodecyl sulfate) was evaluated , Here, a protein isolated from mature leaves and analyzed for proteomics, and also for potential contaminants, widely reported to inhibit proteomics.

The borate buffer / trichloroacetic acid extraction has the lowest protein yield and does not cause any appeal even in a one-dimensional electrophoresis. In contrast, organic solvent / phenol / sodium dodecyl sulfate extraction allowed the highest protein results. In addition, during proteomics, solvent / phenol / sodium dodecyl sulfate extracted organic protein completed the maximum number (144) spots. Further, when the protein is evaluated for contaminants, a significant (77-95%) in the nucleic acid, phenol, and sugar seen with improvements in the extraction procedure. accumulated data suggest that the organic solvent / phenol / sodium dodecyl sulfate extraction is the most effective protocol for the isolation of proteins for proteomics of Shorea robusta and can be used for plants that have a similar set hipokotil contaminants.Excised from developing soybean (Glycine max (L.) Merr. cv. Jack) are cultured on agar-solidified media until callus is formed.

The callus is then propagated in liquid medium until stable, relatively uniform, finely divided suspension culture is obtained. Cells are usually transferred to fresh medium at intervals of 7 days. Cultures were harvested by filtration five days (early log phase) or eight days (the end of the log phase) after the transfer. In order to evaluate the dynamic changes, both intracellular and extracellular proteins were analyzed by two-dimensional difference gel electrophoresis.

Extrapolating a Gaussian Novel Approach for 2-D Gel Electrophoresis Protein Spots Saturated.

Analysis of the images obtained from the electrophoresis two-dimensional (2-D GE) is a topic very important in bioinformatics research, because commercial software and academic currently available have proven to be really effective or fully automated, often requiring revision of the manual and computer repairs the resulting match. In this chapter, we present an effective technique for the detection and reconstruction of protein over-saturated. First, the algorithm reveals overexposed areas, where spots might be cut off, and the plateau area caused by spots smeared and overlapped. Next, he reconstructs the correct distribution of pixel values ​​in the overexposed areas and upland areas, using a least-squares fitting two-dimensional Gaussian distribution by the public. pixel correction in place of saturated and smeared enable more accurate protein quantification, provide results more reliable image analysis. The method is validated for processing highly exposed to 2-D images GE, comparing the reconstructed place with appropriate non-saturated image. The results show that the algorithm allows the quantification of the right places.

 Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.
Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

Identification of DNA-binding proteins that interact with the 5′-flanking region of the D-amino acid oxidase genes humans with pull-down assay coupled with a two-dimensional gel electrophoresis and mass spectrometry.

D-amino acid oxidase (DAO) is flavoenzyme that metabolizes D-amino acids and is expected to be a promising therapeutic target of schizophrenia and glioblastoma. The study of DNA-binding protein has generated a lot of information in transcriptional regulation and other biological processes. However, the protein interacts with genes DAO has not been elucidated.

Our assessment of human DAO promoter activity using luciferase reporter system indicated the 5′-flanking region of this gene (-4289 bp from the transcription initiation site) has a regulatory sequence for gene expression, which is governed by a multi-protein complex interacting with the territory. By using the pull-down test coupled with two-dimensional gel electrophoresis and mass spectrometry, we identified six proteins that bind to the 5′-flanking region of the human DAO gene (C2HC zinc finger domain containing protein 1A; histidine-tRNA ligase, cytoplasm; molybdenum protein cofactor biosynthesis; 60S ribosomal protein L37; calponin-1; calmodulin-binding proteins and heterogeneous nuclear ribonucleoprotein A2 / B1).

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

WSHT-liteDNA Universal Horizontal Electrophoresis Cell

HTES1007 set
EUR 259

WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell

HTES1006 set
EUR 278.6

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH

ELE2300 EACH
EUR 1802.25

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH

ELE2302 EACH
EUR 1891.35

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Aminoacetic acid, ?For electrophoresis

GRM2233-100G 1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis

Aminoacetic acid, ?For electrophoresis

GRM2233-500G 1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Agarose, medium EEO ?For electrophoresis

RM6249-100G 1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-10G 1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-1KG 1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-25G 1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis

Chiller for Electrophoresis systems - EACH

CSLCHILLER EACH
EUR 7642.35

Electrophoresis baffle for Life mini tank

HTDB03 pcs
EUR 5.6

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

Electrophoresis Buffer (10×)

E-BC-R331-100mL 100mL
EUR 15

Electrophoresis Buffer (10×)

E-BC-R331-200mL 200mL
EUR 20

Electrophoresis Buffer (10×)

E-BC-R331-500mL 500mL
EUR 25

Electrophoresis Buffer (10×)

E-BC-R331-each each Ask for price

Electrophoresis baffle for Life Technology Novex

HTDB02 pcs
EUR 2.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

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EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

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EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-1 500 mL
EUR 74.66

Tris-HEPES-SDS Buffer 20X for Electrophoresis

20840057-2 1 L
EUR 139.51

TEMED, electrophoresis grade

RM10358-100G 1 unit
EUR 301.18
Description: TEMED, electrophoresis grade

electrophoresis power supply

EV245 ea
EUR 574.8

Electrophoresis Power Supply

LA690-1NO 1 unit
EUR 432.84
Description: Electrophoresis Power Supply

Electrophoresis baffle for Bio-Rad Mini-PROTEAN

HTDB01 pcs
EUR 2.1

Agarose Gel Electrophoresis Kit

MB570-10PR 1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit

10X MOPS Electrophoresis Buffer

ML050-500ML 1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

E1101 1 each
EUR 636.38

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

miniONE Electrophoresis System - EACH

ELE3400 EACH
EUR 777.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Alkaline Hemoglobin Electrophoresis Kit

MBP001-112RUNS 1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP001-224RUNS 1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP008-224RUNS 1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Paper Electrophoresis Kit (Cell

MBP009-20RUNS 1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell

WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

LA1070-1NO 1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

Electrophoresis Power Supply (2 termina

LA842-1NO 1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina

Cellulose Acetate Electrophoresis System

LA1027-1NO 1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System

WSHT-Basic Electrophoresis Power Supply

HTES1001 set
EUR 557.2

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

HiPer® Agarose Gel Electrophoresis Teach

HTBM001-10PR 1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

WSHT-Universal Electrophoresis Power Supply

HTES1003 set
EUR 784

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

WSHT-miniPRO4 Mini Vertical Electrophoresis Cell

HTES1009 set
EUR 795.2

WSHT-miniPRO2 Mini Vertical Electrophoresis Cell

HTES1010 set
EUR 516.6

WSHT-easyPRO4 Mini Vertical Electrophoresis Cell

HTES1011 set
EUR 837.2

WSHT-easyPRO2 Mini Vertical Electrophoresis Cell

HTES1012 set
EUR 544.6

WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell

HTES1013 set
EUR 602

WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell

HTES1014 set
EUR 448

Gel Electrophoresis System with Time Control, 220-240 V

Q-EP-2114 1
EUR 1180

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH

ELE1111 EACH
EUR 961.2

WSHT-Multi-Functional Electrophoresis Power Supply

HTES1004 set
EUR 896

Gel Electrophoresis System with Muticaster, 100-120 V Input

Q-EP-2034 1
EUR 1715

Gel Electrophoresis System with Muticaster, 220-240 V Input

Q-EP-2056 1
EUR 2125

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell

HTES2001-P set
EUR 459.2

Gel Electrophoresis System with Time Control, 100-120 V Input

Q-EP-2100 1
EUR 1385

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 665.55

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa)

MKPZ010-200mL 200μL
EUR 72

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster)

HTES2001 set
EUR 322

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 727.06

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 757.35

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH

VS10WCES EACH
EUR 1614.21

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade)

E37F007 100-μL
EUR 226.67
Description: Reagents-for-ssDNA-or-RNA-electrophoresis

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade)

E37F001 100-μL
EUR 226.67
Description: Reagents-for-dsDNA-electrophoresis

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 12 x 1.5ml

BV1000-H15 1 PC
EUR 111.76

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 4 x 15ml

BV1000-H150 1 PC
EUR 111.76

Horizontal head, for 2 x 50ml

BV1000-H500 1 PC
EUR 111.76

Horizontal head for 12 x 1.5mL - EACH

MIX7018 EACH
EUR 164.7

Horizontal head for 4 x 15mL - EACH

MIX7020 EACH
EUR 164.7

Horizontal head for 2 x 50mL - EACH

MIX7022 EACH
EUR 164.7

Tube Rack for 7x50ml QuEChERs, horizontal

BV1010-50HQ 1 PC
EUR 43.25

Tube Rack for 7x50mL QuEChERs horizontal - EACH

MIX7044 EACH
EUR 64.8

HORIZONTAL TANK KIT

IB52200 -
EUR 55.88

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

TOOL REST, HORIZONTAL BAR

I4013 1EA
EUR 14.06

IBI QS710 HORIZONTAL UNIT

IB51000 7CM X 10CM FORMAT
EUR 509.62

IBI MP1015 HORIZONTAL UNIT

IB53000 10CM X 15CM FORMAT
EUR 675.93

IBI HR2025 HORIZONTAL UNIT

IB56000 20CM X 25CM FORMAT
EUR 877.32

IBI HR2525 HORIZONTAL UNIT

IB57000 25CM X 25CM FORMAT
EUR 965.82

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 381.6

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 595.2

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 930

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 1030.8

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 422.4

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 686.4

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 727.2

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 747.6

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 625.2

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 757.2

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 930

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

Horizontal shelf for freezer capacity for 16 boxes height 50 mm - EACH

DD37581 EACH
EUR 518.4

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

Pipette Tray PVC Horizontal - EACH

PIP5500 EACH
EUR 49.95

Horizontal 50mL Tube Holder - EACH

MIX5088 EACH
EUR 198.45

INSTRUMENT REST, HORIZONTAL SLOTTED

I556 1EA
EUR 19.7

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 422.4

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 433.2

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 433.2

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 939.6

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 939.6

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 939.6

Horizontal orientator 384 wells - PK2

DD53423 PK2
EUR 87.75

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

HS 260 Basic Horizontal Shaker - EACH

3066602 EACH
EUR 3917.7

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

Axygen Horizontal Gel Box 20Cm - EACH

HGB-20 EACH
EUR 1012.5

These preliminary results will contribute to advances in the understanding of the potential factors associated with regulatory mechanisms DAO expression.Detailed step-by-step method for protein separation techniques based on capillary electrophoresis (CE) are described in this chapter. Focus is placed on two techniques, capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF). Basically CGE gel electrophoresis, performed in the capillaries, where hydrogels are used as the sieving matrix for proteins or peptides separated by size.

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