Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

protein extraction for two-dimensional electrophoresis of network recalcitrant species is quite problematic and challenging because of low protein content and a high abundance of contaminants. Proteomics in Shorea robusta is almost impossible due to lack of preparation procedures suitable protein. To establish a protocol for extracting proteins effectively suitable for electrophoresis two-dimensional in Shorea robusta, four procedures (borate buffer / extraction trichloroacetic acid, organic solvents / precipitation trichloroacetic acid, sucrose / Tris / phenol, and an organic solvent / phenol / sodium dodecyl sulfate) was evaluated , Here, a protein isolated from mature leaves and analyzed for proteomics, and also for potential contaminants, widely reported to inhibit proteomics.

The borate buffer / trichloroacetic acid extraction has the lowest protein yield and does not cause any appeal even in a one-dimensional electrophoresis. In contrast, organic solvent / phenol / sodium dodecyl sulfate extraction allowed the highest protein results. In addition, during proteomics, solvent / phenol / sodium dodecyl sulfate extracted organic protein completed the maximum number (144) spots. Further, when the protein is evaluated for contaminants, a significant (77-95%) in the nucleic acid, phenol, and sugar seen with improvements in the extraction procedure. accumulated data suggest that the organic solvent / phenol / sodium dodecyl sulfate extraction is the most effective protocol for the isolation of proteins for proteomics of Shorea robusta and can be used for plants that have a similar set hipokotil contaminants.Excised from developing soybean (Glycine max (L.) Merr. cv. Jack) are cultured on agar-solidified media until callus is formed.

The callus is then propagated in liquid medium until stable, relatively uniform, finely divided suspension culture is obtained. Cells are usually transferred to fresh medium at intervals of 7 days. Cultures were harvested by filtration five days (early log phase) or eight days (the end of the log phase) after the transfer. In order to evaluate the dynamic changes, both intracellular and extracellular proteins were analyzed by two-dimensional difference gel electrophoresis.

Extrapolating a Gaussian Novel Approach for 2-D Gel Electrophoresis Protein Spots Saturated.

Analysis of the images obtained from the electrophoresis two-dimensional (2-D GE) is a topic very important in bioinformatics research, because commercial software and academic currently available have proven to be really effective or fully automated, often requiring revision of the manual and computer repairs the resulting match. In this chapter, we present an effective technique for the detection and reconstruction of protein over-saturated. First, the algorithm reveals overexposed areas, where spots might be cut off, and the plateau area caused by spots smeared and overlapped. Next, he reconstructs the correct distribution of pixel values ​​in the overexposed areas and upland areas, using a least-squares fitting two-dimensional Gaussian distribution by the public. pixel correction in place of saturated and smeared enable more accurate protein quantification, provide results more reliable image analysis. The method is validated for processing highly exposed to 2-D images GE, comparing the reconstructed place with appropriate non-saturated image. The results show that the algorithm allows the quantification of the right places.

 Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.
Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

Identification of DNA-binding proteins that interact with the 5′-flanking region of the D-amino acid oxidase genes humans with pull-down assay coupled with a two-dimensional gel electrophoresis and mass spectrometry.

D-amino acid oxidase (DAO) is flavoenzyme that metabolizes D-amino acids and is expected to be a promising therapeutic target of schizophrenia and glioblastoma. The study of DNA-binding protein has generated a lot of information in transcriptional regulation and other biological processes. However, the protein interacts with genes DAO has not been elucidated.

Our assessment of human DAO promoter activity using luciferase reporter system indicated the 5′-flanking region of this gene (-4289 bp from the transcription initiation site) has a regulatory sequence for gene expression, which is governed by a multi-protein complex interacting with the territory. By using the pull-down test coupled with two-dimensional gel electrophoresis and mass spectrometry, we identified six proteins that bind to the 5′-flanking region of the human DAO gene (C2HC zinc finger domain containing protein 1A; histidine-tRNA ligase, cytoplasm; molybdenum protein cofactor biosynthesis; 60S ribosomal protein L37; calponin-1; calmodulin-binding proteins and heterogeneous nuclear ribonucleoprotein A2 / B1).

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

Ohaus Tower Kit For Explorer

BAL5154 EACH
EUR 84.36

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6453.54

Horizontal head

BV1000-H15 1 PC
EUR 161.26

Horizontal head

BV1000-H150 1 PC
EUR 161.26

Horizontal head

BV1000-H500 1 PC
EUR 2813.28

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head for 12 x 1.5mL

MIX7018 EACH
EUR 121.98

Horizontal head for 4 x 15mL

MIX7020 EACH
EUR 121.98

Horizontal head for 2 x 50mL

MIX7022 EACH
EUR 121.98

Tube Rack for 7x50mL QuEChERs horizontal

MIX7044 EACH
EUR 47.54

Clear Universal Reload Box for Tower Pack Tips

F167100 EACH
EUR 13.68

Horizontal MicroLoops E

M8-L18SP-15H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

Horizontal MicroLoops E

M8-L18SP-50H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

electrophoresis power supply

EV245 ea
EUR 574.8

miniONE Electrophoresis System

ELE3400 EACH
EUR 624.72

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

Horizontal 50mL Tube Holder

MIX5088 EACH
EUR 144.78

Horizontal orientator 384 wells

DD53423 PK2
EUR 63.84

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

Pipette Tray PVC Horizontal

PIP5500 EACH
EUR 38.76

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Azlon Bottles HPE Heavy Duty Square Graduated Wide Neck

BGE342P PK5
EUR 62.36

Horizontal shelf for freezer capacity for 16 boxes height 50 mm

DD37581 EACH
EUR 352.26

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1448.94

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Tower Pack D10 Refill Of 960 Tips

F167101 PK960
EUR 46.74

Tower Pack DL10 Refill Of 960 Tips

F167102 PK960
EUR 46.74

Tower Pack D200 Refill Of 960 Tips

F167103 PK960
EUR 41.04

Tower Pack D1000 Refill Of 672 Tips

F167104 PK672
EUR 33.06

Tower Pack D300 Refill Of 960 Tips

F167105 PK960
EUR 41.04

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 686.4

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 727.2

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 747.6

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 939.6

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 939.6

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 939.6

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 595.2

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 625.2

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 757.2

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 930

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 930

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 1030.8

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 381.6

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 422.4

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 433.2

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 433.2

6 x 15ml horizontal rotisserie

R4040-HZ15 1 PC
EUR 127.76

6 x 50ml horizontal rotisserie

R4040-HZ50 1 PC
EUR 197.63

HS 260 Basic Horizontal Shaker

3066602 EACH
EUR 3308.28

Horizontal Plastic Clip Microtube Holder

MIX5096 EACH
EUR 173.28

Genie Horizontal Orbital Shaker Attachments

MIX5321 EACH
EUR 523.26

Axygen Horizontal Gel Box 20Cm

HGB-20 EACH
EUR 776.34

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 128.4

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1520.76

Tower Pack Sterile D10ST Refill Of 960 Tips

F167201 PK960
EUR 59.28

Tower Pack Sterile DL10ST Refill Of 960 Tips

F167202 PK960
EUR 59.28

Tower Pack Sterile D200ST Refill Of 960 Tips

F167203 PK960
EUR 53.58

Tower Pack Sterile D1000ST Refill Of 960 Tips

F167204 PK672
EUR 41.04

Tower Pack Sterile D300ST Refill Of 960 Tips

F167205 PK960
EUR 53.58

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 1132.8

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 422.4

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 139.2

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 1132.8

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 496.8

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

6 x 15mL horizontal rotisserie 1 ea

MIX7080 EACH
EUR 76.38

6 x 50mL horizontal rotisserie 1 ea

MIX7082 EACH
EUR 76.38

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
EUR 189.6

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 594.78

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 719.34

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

MAGic Clamp Tube Rack 4 x 15mL tubes horizontal

MIX7174 EACH
EUR 133.38

MAGic Clamp Tube Rack 2 x 50mL tubes horizontal

MIX7176 EACH
EUR 133.38

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E

E1101-SK-E 1 PC
EUR 642.64

MAGic Clamp Tube Rack 12 x 1.5/2.0mL tubes horizontal

MIX7172 EACH
EUR 133.38

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1252.86

Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts

M8-L18SP-HA1 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts; mounted on 18 mm rods

Horizontal outer dims 415h x 140w x 560d with 11 apertures of approx 37mm

AH17911 EACH
EUR 149.34

Horizontal outer dims 415h x 140w x 560d with 6 apertures of approx 67mm

AH1796UT EACH
EUR 168.72

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7
EUR 892.8

Holder for water tank for mounting

CHA4068 EACH
EUR 108.3

Sample comb for batching for AE-6110

2398313 unit
EUR 120
Description: (90mm・r efer ence at t ached・1.5mm t i ck ness)

Adaptor for flexible plates, accessory for AgileSealer?

APS-200-FP
EUR 421.2

Loading step for Ensign for Rodwell autoclaves

AUT1350 EACH
EUR 621.3

Discard System for Genesis for Rodwell autoclaves

AUT2000 EACH
EUR 979.26

FOR Filter for ChemFAST 09 Top/Elite

CAB1460 EACH
EUR 672.6

Falcon Cell Companion For For 12 Well Plate

353503 PK50
EUR 158.46

Automatic FillDrain for Clean Steam for Rodwell autoclaves

AUT1351 EACH
EUR 2211.6

Marking labels for microplates (on sheets for printing)

DD53080 PK3120
EUR 131.1

FOR Filter for ChemFAST 06/12 Top/Elite

CAB1442 EACH
EUR 864.12

Data cable for RS232 connection for Osmomat 3000

WAT4748 EACH
EUR 144.78

I.S. for Haloperidol

50R-R1854 50 mg
EUR 289.2
Description: Haloperidol chemical reference substance

TMB for Microarrays

42R-1004 500 ml
EUR 424.8
Description: Ultra sensitive TMB Peroxidase Substrate ready to use in immunoarrays.

pNPP for ELISA

42R-1005 500 ml
EUR 424.8

TMB for Precipitation

42R-TB100x 1000 ml
EUR 645.6
Description: High sensitivity TMB Substrate for Precipitation assays

TMB for Histochemistry

42R-TB103 500 ml
EUR 392.4
Description: High sensitivity TMB Substrate for Histochemistry assays

For-Ala-OH

5-02335 25g Ask for price

For-Ala-OH

5-02336 100g Ask for price

For-Val-OH

5-03697 25g Ask for price

For-Val-OH

5-03698 100g Ask for price

Stand for D1000

BCM1237 1 pcs, 1 UNIT
EUR 668.04

Stand for D1000

D1000-ST 1 PC
EUR 669.95

For-Ala-OMe

E-1845.0005 5.0g
EUR 240
Description: Sum Formula: C5H9NO3; CAS# [32221-83-3]

For-Ala-OMe

E-1845.0025 25.0g
EUR 849.6
Description: Sum Formula: C5H9NO3; CAS# [32221-83-3]

For-Met-OH

E-1850.0005 5.0g
EUR 151.2
Description: Sum Formula: C6H11NO3S; CAS# [4289-98-9]

For-Met-OH

E-1850.0025 25.0g
EUR 501.6
Description: Sum Formula: C6H11NO3S; CAS# [4289-98-9]

For-Val-OH

E-1855.0005 5.0g
EUR 138
Description: Sum Formula: C6H11NO3; CAS# [4289-97-8]

For-Val-OH

E-1855.0025 25.0g
EUR 428.4
Description: Sum Formula: C6H11NO3; CAS# [4289-97-8]

For-Phe-OH

E-2690.0005 5.0g
EUR 138
Description: Sum Formula: C10H11NO3; CAS# [13200-85-6]

For-Phe-OH

E-2690.0025 25.0g
EUR 385.2
Description: Sum Formula: C10H11NO3; CAS# [13200-85-6]

For-Phe-OMe

E-2825.0005 5.0g
EUR 138
Description: Sum Formula: C11H13NO3; CAS# [2311-21-9]

For-Phe-OMe

E-2825.0025 25.0g
EUR 370.8
Description: Sum Formula: C11H13NO3; CAS# [2311-21-9]

Hematoxylin, For Automation

HAQ500 500 ml
EUR 85.2

Hematoxylin, For Automation

HAQ999 1000 ml
EUR 102

gelcaster for sequencer

ESEQ1000-CAST ea
EUR 524.4

Antibody for CD9

SPC-1272D 0.1ml
EUR 376.8
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is unconjugated.

Antibody for CD9

SPC-1272D-A390 0.1ml
EUR 433.2
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 390.

Antibody for CD9

SPC-1272D-A488 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 488.

Antibody for CD9

SPC-1272D-A565 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 565.

Antibody for CD9

SPC-1272D-A594 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 594.

Antibody for CD9

SPC-1272D-A633 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 633.

Antibody for CD9

SPC-1272D-A655 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 655.

Antibody for CD9

SPC-1272D-A680 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 680.

Antibody for CD9

SPC-1272D-A700 0.1ml
EUR 432
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to ATTO 700.

Antibody for CD9

SPC-1272D-ALP 0.1ml
EUR 426
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to Alkaline Phosphatase.

Antibody for CD9

SPC-1272D-APC 0.1ml
EUR 430.8
Description: A polyclonal antibody for CD9 from Human | Mouse | Rat. The antibody is produced in rabbit after immunization with A synthesized peptide. The Antibody is tested and validated for WB, IHC, ICC/IF assays with the following recommended dilutions: WB (1:1000), IHC (1:200), ICC/IF (1:200). This CD9 antibody is conjugated to APC .

These preliminary results will contribute to advances in the understanding of the potential factors associated with regulatory mechanisms DAO expression.Detailed step-by-step method for protein separation techniques based on capillary electrophoresis (CE) are described in this chapter. Focus is placed on two techniques, capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF). Basically CGE gel electrophoresis, performed in the capillaries, where hydrogels are used as the sieving matrix for proteins or peptides separated by size.

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