Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

protein extraction for two-dimensional electrophoresis of network recalcitrant species is quite problematic and challenging because of low protein content and a high abundance of contaminants. Proteomics in Shorea robusta is almost impossible due to lack of preparation procedures suitable protein. To establish a protocol for extracting proteins effectively suitable for electrophoresis two-dimensional in Shorea robusta, four procedures (borate buffer / extraction trichloroacetic acid, organic solvents / precipitation trichloroacetic acid, sucrose / Tris / phenol, and an organic solvent / phenol / sodium dodecyl sulfate) was evaluated , Here, a protein isolated from mature leaves and analyzed for proteomics, and also for potential contaminants, widely reported to inhibit proteomics.

The borate buffer / trichloroacetic acid extraction has the lowest protein yield and does not cause any appeal even in a one-dimensional electrophoresis. In contrast, organic solvent / phenol / sodium dodecyl sulfate extraction allowed the highest protein results. In addition, during proteomics, solvent / phenol / sodium dodecyl sulfate extracted organic protein completed the maximum number (144) spots. Further, when the protein is evaluated for contaminants, a significant (77-95%) in the nucleic acid, phenol, and sugar seen with improvements in the extraction procedure. accumulated data suggest that the organic solvent / phenol / sodium dodecyl sulfate extraction is the most effective protocol for the isolation of proteins for proteomics of Shorea robusta and can be used for plants that have a similar set hipokotil contaminants.Excised from developing soybean (Glycine max (L.) Merr. cv. Jack) are cultured on agar-solidified media until callus is formed.

The callus is then propagated in liquid medium until stable, relatively uniform, finely divided suspension culture is obtained. Cells are usually transferred to fresh medium at intervals of 7 days. Cultures were harvested by filtration five days (early log phase) or eight days (the end of the log phase) after the transfer. In order to evaluate the dynamic changes, both intracellular and extracellular proteins were analyzed by two-dimensional difference gel electrophoresis.

Extrapolating a Gaussian Novel Approach for 2-D Gel Electrophoresis Protein Spots Saturated.

Analysis of the images obtained from the electrophoresis two-dimensional (2-D GE) is a topic very important in bioinformatics research, because commercial software and academic currently available have proven to be really effective or fully automated, often requiring revision of the manual and computer repairs the resulting match. In this chapter, we present an effective technique for the detection and reconstruction of protein over-saturated. First, the algorithm reveals overexposed areas, where spots might be cut off, and the plateau area caused by spots smeared and overlapped. Next, he reconstructs the correct distribution of pixel values ​​in the overexposed areas and upland areas, using a least-squares fitting two-dimensional Gaussian distribution by the public. pixel correction in place of saturated and smeared enable more accurate protein quantification, provide results more reliable image analysis. The method is validated for processing highly exposed to 2-D images GE, comparing the reconstructed place with appropriate non-saturated image. The results show that the algorithm allows the quantification of the right places.

 Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.
Efficient extraction of proteins from recalcitrant plant tissue for subsequent analysis by two-dimensional gel electrophoresis.

Identification of DNA-binding proteins that interact with the 5′-flanking region of the D-amino acid oxidase genes humans with pull-down assay coupled with a two-dimensional gel electrophoresis and mass spectrometry.

D-amino acid oxidase (DAO) is flavoenzyme that metabolizes D-amino acids and is expected to be a promising therapeutic target of schizophrenia and glioblastoma. The study of DNA-binding protein has generated a lot of information in transcriptional regulation and other biological processes. However, the protein interacts with genes DAO has not been elucidated.

Our assessment of human DAO promoter activity using luciferase reporter system indicated the 5′-flanking region of this gene (-4289 bp from the transcription initiation site) has a regulatory sequence for gene expression, which is governed by a multi-protein complex interacting with the territory. By using the pull-down test coupled with two-dimensional gel electrophoresis and mass spectrometry, we identified six proteins that bind to the 5′-flanking region of the human DAO gene (C2HC zinc finger domain containing protein 1A; histidine-tRNA ligase, cytoplasm; molybdenum protein cofactor biosynthesis; 60S ribosomal protein L37; calponin-1; calmodulin-binding proteins and heterogeneous nuclear ribonucleoprotein A2 / B1).

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1602

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

electrophoresis power supply

EV245 ea
EUR 574.8

miniONE Electrophoresis System

ELE3400 EACH
EUR 691.2

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

myGel Mini Electrophoresis System

E1101 1 each
EUR 623.9

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well)

EDK614 1 UNIT
EUR 520.92

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

Horizontal head, for 12 x 1.5ml

BV1000-H15 1 PC
EUR 108.5

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head for 12 x 1.5mL

MIX7018 EACH
EUR 146.4

Horizontal head, for 4 x 15ml

BV1000-H150 1 PC
EUR 108.5

Horizontal head, for 2 x 50ml

BV1000-H500 1 PC
EUR 108.5

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head for 4 x 15mL

MIX7020 EACH
EUR 146.4

Horizontal head for 2 x 50mL

MIX7022 EACH
EUR 146.4

Tube Rack for 7x50ml QuEChERs, horizontal

BV1010-50HQ 1 PC
EUR 42.4

Tube Rack for 7x50mL QuEChERs horizontal

MIX7044 EACH
EUR 57.6

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

Horizontal 50mL Tube Holder

MIX5088 EACH
EUR 176.4

Pipette Tray PVC Horizontal

PIP5500 EACH
EUR 44.4

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

mini horizontal unit 7x10 cm

EHS1102-SYS ea
EUR 381.6

midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

mini horizontal unit 9x11 cm

EHS3200-SYS ea
EUR 595.2

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
EUR 930

wide horizontal unit 23.5x25 cm

EHS3610-SYS ea
EUR 1030.8

Horizontal orientator 384 wells

DD53423 PK2
EUR 78

midi horizontal unit 10x10 cm

EHS1202-SYS ea
EUR 422.4

maxi horizontal unit 20x10 cm

EHS1401-SYS ea
EUR 686.4

maxi horizontal unit 20x20 cm

EHS1402-SYS ea
EUR 727.2

maxi horizontal unit 20x25 cm

EHS1403-SYS ea
EUR 747.6

mini horizontal unit 12x14 cm

EHS3300-SYS ea
EUR 625.2

midi horizontal unit 15x15 cm

EHS3400-SYS ea
EUR 757.2

midi horizontal unit 15x25 cm

EHS3410-SYS ea
EUR 930

midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

Horizontal shelf for freezer capacity for 16 boxes height 50 mm

DD37581 EACH
EUR 460.8

HS 260 Basic Horizontal Shaker

3066602 EACH
EUR 3482.4

Axygen Horizontal Gel Box 20Cm

HGB-20 EACH
EUR 900

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
EUR 422.4

midiplus horizontal unit 15x10 cm

EHS1302-SYS ea
EUR 433.2

midiplus horizontal unit 15x15 cm

EHS1303-SYS ea
EUR 433.2

maxiplus horizontal unit 26x16 cm

EHS1501-SYS ea
EUR 939.6

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
EUR 939.6

maxiplus horizontal unit 26x32 cm

EHS1503-SYS ea
EUR 939.6

midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 1132.8

6 x 15mL horizontal rotisserie 1 ea

MIX7080 EACH
EUR 92.4

6 x 50mL horizontal rotisserie 1 ea

MIX7082 EACH
EUR 92.4

6 x 15ml horizontal rotisserie, 1 ea

R4040-HZ15 1 PC
EUR 68

6 x 50ml horizontal rotisserie, 1 ea

R4040-HZ50 1 PC
EUR 68

Horizontal Plastic Clip Microtube Holder

MIX5096 EACH
EUR 190.8

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 1132.8

Genie Horizontal Orbital Shaker Attachments

MIX5321 EACH
EUR 603.6

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 496.8

MAGic Clamp Tube Rack 4 x 15mL tubes horizontal

MIX7174 EACH
EUR 163.2

MAGic Clamp Tube Rack 2 x 50mL tubes horizontal

MIX7176 EACH
EUR 163.2

MAGic Clamp Tube Rack 12 x 1.5/2.0mL tubes horizontal

MIX7172 EACH
EUR 163.2

6 x 1.5/2.0ml tube holder, pack of 2 (Installed for vertical or horizontal mixing)

R4040-1520 1 PC
EUR 22.3

MAGic Clamp™ Tube Rack, 4 x 15ml tubes, horizontal

H1000-MR-T150H 1 PC
EUR 122

MAGic Clamp™ Tube Rack, 2 x 50ml tubes, horizontal

H1000-MR-T500H 1 PC
EUR 122

MAGic Clamp™ Tube Rack, 12 x 1.5/2.0ml tubes, horizontal

H1000-MR-T15H 1 PC
EUR 122

Horizontal outer dims 415h x 140w x 560d with 11 apertures of approx 37mm

AH17911 EACH
EUR 184.8

Horizontal outer dims 415h x 140w x 560d with 6 apertures of approx 67mm

AH1796UT EACH
EUR 208.8

Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

M8-L18SP-15H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

M8-L18SP-50H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

OPTIONAL HEAD FOR 4X15ML TUBES, HELD HORIZONTALLY

480106 1/pk
EUR 111.6
Description: Lab Equipment; Shakers & Mixers

OPTIONAL HEAD FOR 2X50ML TUBES, HELD HORIZONTALLY

480107 1/pk
EUR 111.6
Description: Lab Equipment; Shakers & Mixers

OPTIONAL HEAD FOR 12X1.5/2.0ML TUBES, HELD HORIZONTALLY

480105 1/pk
EUR 75.6
Description: Lab Equipment; Shakers & Mixers

MicroTools for Measurements: Kit 3; 20 measuring tools with standard tips Horizontal MicroRuler: Five 500 micrometer wide horizontal rulers with 25 micrometer gradations. Vertical MicroRuler: Five 1000 micrometer long vertical rulers with 25 micrometer g

T3-L25-A1 20 TOOLS
EUR 118
Description: MicroTools for Measurements: Kit 3; 20 measuring tools with standard tipsHorizontal MicroRuler: Five 500 micrometer wide horizontal rulers with 25 micrometer gradations.Vertical MicroRuler: Five 1000 micrometer long vertical rulers with 25 micrometer gradations.Large MicroProtractor: Five 1000 micrometer diameter tools for measuring angles and radial distances/dimensions, with 15 degree and 50 micrometer gradations. Small MicroProtractor: Five 320 micrometer diameter tools for measuring angles and radial distances/dimensions, with 30 degree and 20 micrometer gradations.

Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts; mounted on 18 mm rods

M8-L18SP-HA1 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E Assortment - 10 each of 15x150 and 50x500 micron aperture mounts; mounted on 18 mm rods

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-15H-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-15H-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-15H-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-15H-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-15H-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-50H-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-50H-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-50H-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-50H-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-50H-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

B-M8-15H-B1-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

B-M8-15H-B1A-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

B-M8-15H-B3-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

B-M8-15H-B3S-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

B-M8-15H-B5-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 15 x 150 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

B-M8-50H-B1-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

B-M8-50H-B1A-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

B-M8-50H-B3-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

B-M8-50H-B3S-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

B-M8-50H-B5-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E, 20 of 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

B-M8-HA1-B1 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

B-M8-HA1-B1A 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

B-M8-HA1-B3 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3 bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

B-M8-HA1-B3S 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

B-M8-HA1-B5 20 MOUNT/BASE ASSEMBLIES
EUR 249
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5 bases.

Ohaus Tower Kit For Explorer

BAL5154 EACH
EUR 96

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

B-M8-HA1-B1-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

B-M8-HA1-B1A-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B1A-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

B-M8-HA1-B3-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

B-M8-HA1-B3S-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B3S-R reusable bases.

MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

B-M8-HA1-B5-R 20 MOUNT/BASE ASSEMBLIES
EUR 339
Description: MicroLoop E Assembly; box of 20 assemblies, horizontal MicroLoop E assortment, 10 each of 15 x 150 and 50 x 500 micron Mounts on 18mm/SPINE length rods adhered into B5-R reusable bases.

Clear Universal Reload Box for Tower Pack Tips

F167100 EACH
EUR 15.6

SS grid electropolished for size 30

INC8202 EACH
EUR 133.2

SS grid electropolished for HPP750

CHA4054 EACH
EUR 318

SS grid electropolished for sizes 260/400

CHA4052 EACH
EUR 224.4

Tower Pack D10 Refill Of 960 Tips

F167101 PK960
EUR 52.8

Tower Pack DL10 Refill Of 960 Tips

F167102 PK960
EUR 52.8

Tower Pack D200 Refill Of 960 Tips

F167103 PK960
EUR 46.8

Tower Pack D300 Refill Of 960 Tips

F167105 PK960
EUR 46.8

Tower Pack D1000 Refill Of 672 Tips

F167104 PK672
EUR 38.4

Plant Electroporation Buffer for Transfection

GR103051 1 L
EUR 119

These preliminary results will contribute to advances in the understanding of the potential factors associated with regulatory mechanisms DAO expression.Detailed step-by-step method for protein separation techniques based on capillary electrophoresis (CE) are described in this chapter. Focus is placed on two techniques, capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF). Basically CGE gel electrophoresis, performed in the capillaries, where hydrogels are used as the sieving matrix for proteins or peptides separated by size.

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