Serum protein concentrations and protein fractions in clinically healthy Italian Heavy Draft Horses using agarose gel electrophoresis.

serum protein electrophoresis (SPE) reference intervals (gathering) was evaluated in a different horse, but no specific values ​​that will be displayed for the descendants of horses as described previously in other species (dogs, cats), and no research SPE has been done on the draft horse .This study aims to determine the get-together for the SPE in heavy draft horses (Italian heavy draft horses IHDH) living in central Italy. Comparisons between different countries physiological (pregnancy and no pregnancy) and age (foals and adults) are executed.

Blood samples collected from 215 apparently healthy horses (horses, ponies and foals). SPE (total protein, albumin, α1-, α2-, β1-, β2-, and γ-fractions, A / G) were evaluated in Teaching Veterinary Hospital of the University of Perugia. RIs determined using the well described, modern methods and statistical analysis. Normality of distribution was assessed using Anderson-Darling test. Differences between subgroups were evaluated using the Mann-Whitney U AP <0.05 was considered statistically significant for all analyses.Our results indicate that IHDHs have an increase in TPS and α2- it, β1-, β2-, and γ-fraction, and a decrease albumin, α1-globulin, and A / G ratio is compared with data reported in other horses.

We also found that the foal has a significantly higher α1-globulin and albumin concentrations were significantly lowered, and the ratio of A / G compared with those of adult horses.In this study, SPE-togethers using agarose gel has been determined for the first time in a large number of draft horses (represented by IHDH). The results provide a basis for further investigation of the descendants of horses with natural breeding, and the effects of age and physiological state in SPE.

Application of two-dimensional gel electrophoresis technique of protein profiles of black gram varieties India and detection of forgery in black gram based food products using comparative proteomics.

In this study, the protein fingerprint of six varieties of black gram Novel India have been obtained using 2D-GE. The results revealed the presence of thirteen well settled point of the protein in all six varieties. Fingerprint analysis using the PD Quest ™ reveals differential protein expression. In addition, six proteins were found to be uniquely expressed in varieties PDKV Black Gold and TAU-1.

Furthermore, the analysis of 2D patterns of black gram and potential adulterants such as refined flour and white beans by using PD Quest ™ reveals the presence of a protein with a MW of 15.0 kDa, pI 4.89 (refined flour) and MW 21.5 kDa, pI 5, 70 (white beans), which can be considered as a biomarker for their presence in the black gram of food product. This method is sensitive enough to detect forgery at 5% level and managed to detect the presence of potential flour in one gram sample was analyzed for study.

To black papad understand early heat shock (HS) -regulated cellular responses that affect the rice crop tolerance to environmental temperature high, two-dimensional difference gel electrophoresis (2D-DIGE) was conducted to explore the HS-regulated early proteome. Some proteins that show changes in abundance after 1 and 5 minutes of treatment HS identified. HS-regulated proteins were identified early, the abundance of ubiquitin-specific proteases, OsUBP21, and homologous Arabidopsis, AtUBP13, was found to be upregulated by 5 minutes of treatment of HS.

Furthermore, the expression OsUBP21 or AtUBP13 knocked down or out increased tolerance of rice and Arabidopsis plants HS stress, suggesting that the ubiquitin-specific proteases function in regulating plant HS response is conserved between monocots and dicots

 Serum protein concentrations and protein fractions in clinically healthy Italian Heavy Draft Horses using agarose gel electrophoresis.
Serum protein concentrations and protein fractions in clinically healthy Italian Heavy Draft Horses using agarose gel electrophoresis.

quantitative protein expression profiling regional in rat retina after optic nerve transection partial use fluorescence difference gel electrophoresis in two dimensions.

To test the difference between the retinal ganglion cell (RGC) degeneration of primary and secondary, protein expression in the four regions of the retina including superior quadrants temporal, inferior and nose in a mouse model transection of the optic nerve partial (Pont) using 2-D fluorescence difference gel electrophoresis (DIGE ) was investigated.

Unilateral Pont done on the temporal side of the optic nerve of adult Wistar rats RGC loss of separate primary and secondary. quantification of the topography of RGCs labeled by Rbpms antibody and analysis of injury axonal by levels of damage to the optic nerve at 1 week (n = 8) and 8 weeks (n = 15) after Pont showed a loss of RGC early in the temporal region, which is regarded as the primary degeneration of RGC and RGC loss progress in the area of ​​the nose, which is regarded as secondary RGC degeneration. protein expression beginning of each quadrant of the retina (n = 4) at 2 weeks after the Pont compared with the corresponding quadrant in the contralateral control eyes by DIGE. For all comparisons, 24 proteins were expressed differently (> 1.2-fold; P <0.05; ≥3 non-duplicated peptide matches) were identified by mass spectrometry (MS).

maxiplus horizontal unit 26x24 cm

EHS1502-SYS ea
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maxiplus horizontal unit 26x32 cm

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mini horizontal unit 9x11 cm

EHS3200-SYS ea
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mini horizontal unit 12x14 cm

EHS3300-SYS ea
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midi horizontal unit 15x15 cm

EHS3400-SYS ea
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midi horizontal unit 15x25 cm

EHS3410-SYS ea
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midi horizontal unit 20x25 cm

EHS3500-SYS ea
EUR 980.4

wide horizontal unit 23.5x14 cm

EHS3600-SYS ea
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wide horizontal unit 23.5x25 cm

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EUR 1030.8

mini horizontal unit 7x7 cm

EHS1101-SYS ea
EUR 381.6

mini horizontal unit 7x10 cm

EHS1102-SYS ea
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midi horizontal unit 10x7 cm

EHS1201-SYS ea
EUR 422.4

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EHS1202-SYS ea
EUR 422.4

midiplus horizontal unit 15x7 cm

EHS1301-SYS ea
EUR 433.2

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EHS1302-SYS ea
EUR 433.2

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EHS1303-SYS ea
EUR 433.2

UNIVERSAL POWER SUPPLY FOR AXYGEN MOTOPET

MOTO-SP-PWR 1/pk
EUR 73.2
Description: Handheld Pipettor Repairs; Liquid Handling Device Repairs

maxi horizontal unit 20x10 & 20x20 cm

EHS1400-SYS ea
EUR 727.2

maxiplus horizontal unit 26x16-24-32cm

EHS1500-SYS ea
EUR 1132.8

mini horizontal unit 7x7 & 7x10 cm

EHS1100-SYS ea
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midi horizontal unit 10x7 & 10x10 cm

EHS1200-SYS ea
EUR 453.6

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS ea
EUR 1132.8

midiplus horizontal unit 15x7-10-15 cm

EHS1300-SYS ea
EUR 496.8

CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus UK Power Supply

4250-050-ESK-PS3 1 Kit
EUR 4597.68

CometAssay Electrophoresis Starter Kit plus SZ Power Supply

4250-050-ESK-PS4 1 Kit
EUR 4597.68

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

Slide storing sys w support feet

MIC4526 EACH
EUR 599.64

LABeler Lab Printer w/ 115V power supply

L9010 1/pack
EUR 224.76
Description: LABeler Hand Held Labeler

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 719.34

LinkLabel BlueTooth Enabled Label Printer w/ 115-230V power supply

L3000 1/pack
EUR 350.63
Description: LinkLabel Bluetooth Enabled Labeler

Horizontal head

BV1000-H15 1 PC
EUR 161.26

Horizontal head

BV1000-H150 1 PC
EUR 161.26

Horizontal head

BV1000-H500 1 PC
EUR 2813.28

Horizontal MicroLoops E

M8-L18SP-15H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods

Horizontal MicroLoops E

M8-L18SP-50H 20 MOUNTS
EUR 112
Description: Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

miniONE Electrophoresis System

ELE3400 EACH
EUR 624.72

Four Source Power Supply 

EDK621 1 UNIT
EUR 821.08

Eps 301 Power Supply

18113001 EACH
EUR 762.66

Consort Power Supply EV2230

ELE9104 EACH
EUR 1185.6

Consort Power Supply EV2310

ELE9106 EACH
EUR 1077.3

Consort Power Supply EV2320

ELE9108 EACH
EUR 1185.6

Consort Power Supply EV2650

ELE9110 EACH
EUR 1077.3

Consort Power Supply EV3020

ELE9112 EACH
EUR 1553.82

Consort Power Supply EV3150

ELE9114 EACH
EUR 1553.82

Consort Power Supply EV3330

ELE9116 EACH
EUR 2330.16

Consort Power Supply EV3610

ELE9118 EACH
EUR 1553.82

Consort Power Supply EV3620

ELE9120 EACH
EUR 2330.16

Horizontal 50mL Tube Holder

MIX5088 EACH
EUR 144.78

Horizontal orientator 384 wells

DD53423 PK2
EUR 63.84

Midi Horizontal Gel Unit

ELE8120 EACH
EUR 630.42

Pipette Tray PVC Horizontal

PIP5500 EACH
EUR 38.76

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6453.54

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1448.94

Dual Source 150 Power Supply 

EDK620 1 UNIT
EUR 445.75

Power Supply for Spectrawave S800

80211538 EACH
EUR 88.92

Plastic bottles 250ml supply/waste

WAT4842 PK2
EUR 144.78

6 x 15ml horizontal rotisserie

R4040-HZ15 1 PC
EUR 127.76

6 x 50ml horizontal rotisserie

R4040-HZ50 1 PC
EUR 197.63

HS 260 Basic Horizontal Shaker

3066602 EACH
EUR 3308.28

Horizontal Plastic Clip Microtube Holder

MIX5096 EACH
EUR 173.28

Genie Horizontal Orbital Shaker Attachments

MIX5321 EACH
EUR 523.26

Axygen Horizontal Gel Box 20Cm

HGB-20 EACH
EUR 776.34

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 128.4

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1520.76

Mini-6 Gel Documentation Sys

ELE6580 EACH
EUR 23627.64

Mini-9 Gel Documentation Sys

ELE6582 EACH
EUR 24708.36

myVolt™ Mini power supply, 115V

BM0294 Ea
EUR 368.4

myVolt™ Mini power supply, 230V

BM0295 Ea
EUR 368.4

myVolt™ Touch power supply, 115V

BM0296 Ea
EUR 861.6

myVolt™ Touch power supply, 230V

BM0297 Ea
EUR 861.6

Power supply for LABeler Lab Printer

L9010-PS 1/pack
EUR 100.32
Description: Power supply for Labeler

ENDURO™ 250V Power Supply , 120V

LE0203-120 Ea
EUR 1923.6

ENDURO™ 250V Power Supply , 230V

LE0203-230 Ea
EUR 1923.6

ENDURO™ 300V Power Supply , 120V

LE0303-120 Ea
EUR 1437.6

myVolt™ Mini Power Supply,115V

LE1007 Ea
EUR 368.4

myVolt™ Mini Power Supply, 230V

LE1008 Ea
EUR 368.4

myVolt™ Touch power supply, 115V

LE1009 Ea
EUR 861.6

myVolt™ Touch power supply, 220V

LE1010 Ea
EUR 861.6

Accuris myVolt Mini Power Supply 230V

ELE1534 EACH
EUR 270.18

Accuris myVolt Touch Power Supply 230V

ELE1535 EACH
EUR 719.34

Kinesis Power Supply for EV/LabPro

CHR2495 EACH
EUR 168.72

Power supply unit with ventilator complete

WAT4774 EACH
EUR 831.06

Sodium Hydroxide 30% w/w (40% w/v)

S30WW 25L
EUR 229.14

Sodium Hydroxide 30% w/w (40% w/v)

S30WW1 1L
EUR 51.3

Horizontal head, for 12 x 1.5ml

BCM1604 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 4 x 15ml

BCM1605 1 pcs, 1 UNIT
EUR 200.68

Horizontal head, for 2 x 50ml

BCM1606 1 pcs, 1 UNIT
EUR 200.68

Horizontal head for 12 x 1.5mL

MIX7018 EACH
EUR 121.98

Horizontal head for 4 x 15mL

MIX7020 EACH
EUR 121.98

Horizontal head for 2 x 50mL

MIX7022 EACH
EUR 121.98

Tube Rack for 7x50mL QuEChERs horizontal

MIX7044 EACH
EUR 47.54

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 139.2

Nitrogen Std 0.1% w/w

NH01WW01 100ML
EUR 38.76

Hydrochloric Acid 30% w/w

H30WW 25L
EUR 153.9

25% w/w Sodium Hydroxide

S25WW1 1L
EUR 25.08

Arium Pro Basic Water Sys. Bench

WAT4242 EACH
EUR 5503.92

Arium Pro Basic Water Sys. Wall

WAT4243 EACH
EUR 5503.92

Arium Pro DI Water Sys. Bench

WAT4244 EACH
EUR 7350.72

Arium Pro DI Water Sys. Wall

WAT4245 EACH
EUR 7350.72

Arium Pro UF Water Sys. Bench

WAT4247 EACH
EUR 9093.78

Arium Pro UF Water Sys. Wall

WAT4248 EACH
EUR 9094.92

Arium Pro UV Water Sys. Bench

WAT4250 EACH
EUR 8772.3

Arium Pro UV Water Sys. Wall

WAT4251 EACH
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Arium Pro VF Water Sys. Bench

WAT4256 EACH
EUR 10184.76

Arium Pro VF Water Sys. Wall

WAT4257 EACH
EUR 10184.76

RIOS/ELIX-L SYS-WALL BRACKET

ZLX0WMBKT EACH
EUR 346.56

Kjeldahl Reagent 30% w/w (40% w/v) NaOH

S30WW5 5L
EUR 54.72

Kjeldahl Reagent 30% w/w (40% w/v) NaOH

S30WWLN 5L
EUR 92.34

Axygen 250V High Amperage Power Supply - 115V

PS250HA EACH
EUR 1088.7

Axygen 250V High Amperage Power Supply - 230V

PS250HA-230V EACH
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Axygen 300V General Purpose Power Supply - 115V

PS300GP EACH
EUR 572.28

Axygen 300V General Purpose Power Supply - 230V

PS300GP-230V EACH
EUR 572.28

Nitrogen Std Solution 0.64% w/w

NH064WW01 100ML
EUR 171

Labsit4 Leather w castors/ring W

FUR3533 EACH
EUR 286.14

Hydrochloric Acid 10% w/w Solution

H10WW5 5L
EUR 110.58

Sodium Lauryl Sulphate 13% w/w

SLS1301 1L
EUR 247.38

Acetone 0.1% w/w in Water

RACET0010 500ML
EUR 127.68

Acetone 0.5% w/w in Water

RACET0050 500ML
EUR 128.82

Acetone 0.75% w/w in Water

RACET0075 500ML
EUR 128.82

Acetone 1% w/w in Water

RACET0100 500ML
EUR 128.82

AXYGEN® HORIZONTAL GEL BOX, 10 CM

HGB-10 1/pk
EUR 399.6
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 15 CM

HGB-15 1/pk
EUR 583.2
Description: Lab Equipment; Axygen Branded EQ

AXYGEN® HORIZONTAL GEL BOX, 7 CM

HGB-7 1/pk
EUR 343.2
Description: Lab Equipment; Axygen Branded EQ

6 x 15mL horizontal rotisserie 1 ea

MIX7080 EACH
EUR 76.38

6 x 50mL horizontal rotisserie 1 ea

MIX7082 EACH
EUR 76.38

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 594.78

Interestingly, in the nasal retina, serum albumin and crystallin family members, including αA, αB, βA2, βA3, βB2 and gamma S indicate a stress response that is regulated. Instead, just and βA2 αB crystallin protein altered in temporal quadrant. In the superior and inferior quadrants, βB2 crystallin, keratin type I, S-Arrestin and Lamin-B1 regulated, while the heat shock cognate 71 kDa proteins and heterogeneous nuclear ribonucleoproteins A2 / B1 were downregulated. In short, the use of DIGE followed by MS useful for detecting protein regulation regional early in the retina after optic nerve injury locally.

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