New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Current methods for identification and / or quantification of viral proteins of influenza virus and suffer virosome sample long analysis times, the coverage is limited protein and / or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to achieve a faster and improved characterization and quantification of viral proteins. sample preparation and gel buffer composition were investigated in order to achieve sufficient separation of proteins in a relatively short time.

Preparation of the total sample (reduction and deglycosylation) can be carried out efficiently within two hours. hydrodynamic injection, separation voltage, and temperature-optimized capillary in full factorial design. The latter method has been validated and shown good performance for hemagglutinin fragment 1 (HA1), hemagglutinin fragment 2 (HA2), the matrix protein (M) and nucleoprotein (NP). CGE method allows the identification of different viral strains based on their specific protein profiles. B / Brisbane attenuated virus and virosome samples can be analyzed in a single day.

The results of CGE (titer) is proportional to the radial immune-diffusion (SRID), but this method has the advantage of a much faster time to results. CGE analysis of A / Christchurch from upstream processes demonstrate the applicability of the method to samples of high complexity. CGE method can be used in the same analyte concentration range as the RP-HPLC method, but show the precision and accuracy that is better. Overall, the total analysis time for CGE method is much shorter, which allows the analysis of 100 samples within 4 days instead of 10 days for SRID.

Identification and comparative proteomic studies quail and duck egg white protein using 2-dimensional gel electrophoresis and matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry analysis.

A study of proteomics egg white protein of two species of large poultry, the quail (Coturnix Coturnix) and duck (Anas platyrhynchos), is done by comparison with those of the chicken (Gallus gallus) through 2-dimensional polyacrylamide gels (2-DE ) analysis. By using matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry (MALDI-TOF MS / MS), 29 where protein represents 10 types of protein and 17 where proteins appoint nine proteins have been identified in quail and duck egg white , each.

This report suggests a closer relationship between quail and chicken egg white proteome pattern, while a duck egg white protein distribution on the 2-DE map is much different. In the duck egg whites, some of the major proteins of the famous, such as ovomucoid, clusterin, extracellular fatty acid binding protein precursor (ex-FABP), and prostaglandin D2 synthase (PG D2 synthase), undetectable, while the two main proteins identified as “deleted malignant brain tumors 1 “protein (DMBT1) and vitellogenin-2 was found on a duck in the appropriate range on a map 2-DE gel.

This interspecies diversity may be related to egg white protein function in cellular defense or arrange / support embryo development to adapt to the environment that inhabit or reproduction demand for long-term evolution. The findings of this work will provide insight into the benefits involved in the application in the egg white protein from a variety of sources of eggs, which can lead to advantageous properties of the novel in the food industry or related to human health.

 New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.
New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Two-dimensional polyacrylamide gel electrophoresis Bali banteng (Bos javanicus) seminal plasma proteins and their association with semen quality.

This study evaluated the relationship between Bali banteng (Bos javanicus) protein seminal plasma and semen quality parameters are different. semen samples from 10 adult bulls Bali evaluated for semen parameters of conventional (motility general, viability, and normal morphology), sperm function (acrosome reaction, the rate of penetration of sperm, the index of penetration of sperm), the kinetics of sperm (computer aided analysis parameters of semen such as the speed of sperm), and morphology of sperm (acrosome and membrane integrity).

Frozen-thawed semen with a higher sperm motility, viability, acrosome integrity and membrane integrity (P <0.05) was consistently higher in the acrosome reaction and sperm penetration test. Three bulls showed the highest, four bulls secondary display, and the remaining three cows lowest showing for all sperm parameters and SPA. seminal plasma proteome maps of high-quality and low-quality Bali cattle were also established. seminal plasma of both high quality and low quality bulls Bali was subjected to two-dimensional SDS-PAGE with isoelectric points ranging from 3 to 10 and a molecular weight of 10-250 kDa. Approximately 116 points detected by Blue Silver stains and spots, 29 have been selected and identified by MALDI-TOF / TOF-MS / MS.

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

Axygen Gel Documentation System

GD-1000 EACH
EUR 5947.38

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Nugenius Gel Docu System LED

ELE0028 EACH
EUR 6692.94

Nugenius Gel Docu System Plus

ELE0030 EACH
EUR 7993.68

Gel Doc System EtBr Filter

ELE2500 EACH
EUR 6733.98

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 594.78

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7
EUR 892.8

AXYGEN® GEL DOCUMENTATION SYSTEM BL

GDBL-1000 1/pk
EUR 9158.4
Description: Lab Equipment; Axygen Branded EQ

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Linear Polyacrylamide (5mg/ml)

S143 1 ml
EUR 48

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E

E1101-SK-E 1 PC
EUR 642.64

Gel and PCR Extraction System (100 preps)

9K-006-0007 100preps
EUR 180.06

Gel and PCR Extraction System (200 Preps)

9K-006-0008 200preps, 200prep
EUR 263.58

Casting Dams, For Enduro 7cm Gel System

LE1000CD-01 Ea
EUR 151.2

Casting Dams, For Enduro 10cm Gel System

LE1000CD-02 Ea
EUR 165.6

Casting Dams, For Enduro 15cm Gel System

LE1000CD-03 Ea
EUR 198

Casting Dams, For Enduro 20cm Gel System

LE1000CD-04 Ea
EUR 230.4

Gbox F3 with LFB Gel Doc System

ELE7592 EACH
EUR 8475.9

G Box Chemi Xrq Gel Doc System

ELE7601 EACH
EUR 14585.16

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1448.94

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1520.76

17-Hydroxyprogesterone (17-OHP) ELISA Kit

DLR-17-OHP-Ge-48T 48T
EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

17-Hydroxyprogesterone (17-OHP) ELISA Kit

DLR-17-OHP-Ge-96T 96T
EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RDR-17-OHP-Ge-48Tests 48 Tests
EUR 585.6

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RDR-17-OHP-Ge-96Tests 96 Tests
EUR 811.2

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RD-17-OHP-Ge-48Tests 48 Tests
EUR 560.4

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RD-17-OHP-Ge-96Tests 96 Tests
EUR 775.2

electrophoresis power supply

EV245 ea
EUR 574.8

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1252.86

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
EUR 153.6

12 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-04 Ea
EUR 153.6

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-05 Ea
EUR 153.6

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 153.6

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-07 Ea
EUR 153.6

16 Well Comb ,2mm Thick For Enduro 10cm Gel System

LE1000C10-08 Ea
EUR 153.6

25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
EUR 153.6

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 153.6

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 157.2

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
EUR 157.2

15 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-03 Ea
EUR 157.2

14 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-04 Ea
EUR 157.2

16 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-05 Ea
EUR 157.2

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-10 Ea
EUR 157.2

20 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-11 Ea
EUR 157.2

20 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-12 Ea
EUR 157.2

20 Well Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-13 Ea
EUR 157.2

16 Well Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-01 Ea
EUR 165.6

25 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-05 Ea
EUR 165.6

30 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-06 Ea
EUR 165.6

50 Well Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-10 Ea
EUR 165.6

10 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-01 Ea
EUR 148.8

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-02 Ea
EUR 148.8

16 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-05 Ea
EUR 148.8

16 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-06 Ea
EUR 148.8

5 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-07 Ea
EUR 148.8

8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
EUR 148.8

8 Well Comb ,1mm Thick For Enduro 7cm Gel System

LE1000C7-09 Ea
EUR 148.8

GEL LOADING TIPS,10UL,FLAT,0.17MM OD,NONSTERILE,RACK,200/400

TGL-10FT-17-R 200/pk
EUR 169.2
Description: Non Filter Tips; Pipette Tips - Axygen

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 222

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1904.4

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-01 Ea
EUR 153.6

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-02 Ea
EUR 153.6

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-09 Ea
EUR 153.6

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-10 Ea
EUR 153.6

16 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-06 Ea
EUR 157.2

16 Well Mc Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-07 Ea
EUR 157.2

18 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-08 Ea
EUR 157.2

18 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-09 Ea
EUR 157.2

28 Well Mc Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-14 Ea
EUR 157.2

28 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-15 Ea
EUR 157.2

30 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-16 Ea
EUR 157.2

20 Well Mc Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-02 Ea
EUR 165.6

20 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-03 Ea
EUR 165.6

20 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-04 Ea
EUR 165.6

40 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-07 Ea
EUR 165.6

40 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-08 Ea
EUR 165.6

40 Well Mc Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-09 Ea
EUR 165.6

12 Well Mc Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-03 Ea
EUR 148.8

12 Well Mc Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-04 Ea
EUR 148.8

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6453.54

Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 927.6
Description: Minimum order quantity: 1 unit of 1EA

FT3 (Free triiodothyronine) ELISA test

17 96T/Box Ask for price
Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine)

Reproductive Tissue Dissociation System 17 (Sertoli), Mouse and Rat

4-20426 ea Ask for price

AXYGEN® GEL DOCUMENTATION SYSTEM BLUE LIGHT CONVERSION SCREEN FOR GD-1000

GD-BLCS 1/pk
EUR 840
Description: Lab Equipment; Axygen Branded EQ

Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 210

TC Gel

CP048-005 500 g
EUR 278.4

TC Gel

CP048-010 1 Kg
EUR 416.4

Gel Tray

2394250 4unit
EUR 324
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
EUR 424.8

Gel Cutter

KS071012-11 12
EUR 106.8
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Gel Filter

KS071012-13 12
EUR 138
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 128.4

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 139.2

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 383.11

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 487.51

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 487.51

Gel tray L

2322112 5unit
EUR 308.4
Description: 2pcs/1set

Gel tray S

2322117 5unit
EUR 308.4
Description: 2pcs/1set

AGAR, HIGH GEL

A01-102IHG-10kg 10 kg
EUR 2662.8

AGAR, HIGH GEL

A01-102IHG-2Kg 2 Kg
EUR 625.2

AGAR, HIGH GEL

A01-102IHG-500g 500 g
EUR 213.6

The majority of the proteins visualized in a two-dimensional map of the seminal plasma were identified. An important group of spots identified are represented binder sperm 1 (BSP1), clusterin, spermadhesin, tissue inhibitor of metalloproteinase 2 (TIMP-2), and phospholipase A2 (PLA2). Another protein found in high abundance including seminal ribonuclease, serum albumin, cationic trypsin, and peptide similar to the β2 -microglobulin. Thus, the reference maps Bali bull seminal plasma proteins have been produced for the first time and can be used to relate changes in the pattern of protein physiopathologic events that can affect the reproductive performance of Bali bull.

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