New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Current methods for identification and / or quantification of viral proteins of influenza virus and suffer virosome sample long analysis times, the coverage is limited protein and / or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to achieve a faster and improved characterization and quantification of viral proteins. sample preparation and gel buffer composition were investigated in order to achieve sufficient separation of proteins in a relatively short time.

Preparation of the total sample (reduction and deglycosylation) can be carried out efficiently within two hours. hydrodynamic injection, separation voltage, and temperature-optimized capillary in full factorial design. The latter method has been validated and shown good performance for hemagglutinin fragment 1 (HA1), hemagglutinin fragment 2 (HA2), the matrix protein (M) and nucleoprotein (NP). CGE method allows the identification of different viral strains based on their specific protein profiles. B / Brisbane attenuated virus and virosome samples can be analyzed in a single day.

The results of CGE (titer) is proportional to the radial immune-diffusion (SRID), but this method has the advantage of a much faster time to results. CGE analysis of A / Christchurch from upstream processes demonstrate the applicability of the method to samples of high complexity. CGE method can be used in the same analyte concentration range as the RP-HPLC method, but show the precision and accuracy that is better. Overall, the total analysis time for CGE method is much shorter, which allows the analysis of 100 samples within 4 days instead of 10 days for SRID.

Identification and comparative proteomic studies quail and duck egg white protein using 2-dimensional gel electrophoresis and matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry analysis.

A study of proteomics egg white protein of two species of large poultry, the quail (Coturnix Coturnix) and duck (Anas platyrhynchos), is done by comparison with those of the chicken (Gallus gallus) through 2-dimensional polyacrylamide gels (2-DE ) analysis. By using matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry (MALDI-TOF MS / MS), 29 where protein represents 10 types of protein and 17 where proteins appoint nine proteins have been identified in quail and duck egg white , each.

This report suggests a closer relationship between quail and chicken egg white proteome pattern, while a duck egg white protein distribution on the 2-DE map is much different. In the duck egg whites, some of the major proteins of the famous, such as ovomucoid, clusterin, extracellular fatty acid binding protein precursor (ex-FABP), and prostaglandin D2 synthase (PG D2 synthase), undetectable, while the two main proteins identified as “deleted malignant brain tumors 1 “protein (DMBT1) and vitellogenin-2 was found on a duck in the appropriate range on a map 2-DE gel.

This interspecies diversity may be related to egg white protein function in cellular defense or arrange / support embryo development to adapt to the environment that inhabit or reproduction demand for long-term evolution. The findings of this work will provide insight into the benefits involved in the application in the egg white protein from a variety of sources of eggs, which can lead to advantageous properties of the novel in the food industry or related to human health.

 New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.
New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Two-dimensional polyacrylamide gel electrophoresis Bali banteng (Bos javanicus) seminal plasma proteins and their association with semen quality.

This study evaluated the relationship between Bali banteng (Bos javanicus) protein seminal plasma and semen quality parameters are different. semen samples from 10 adult bulls Bali evaluated for semen parameters of conventional (motility general, viability, and normal morphology), sperm function (acrosome reaction, the rate of penetration of sperm, the index of penetration of sperm), the kinetics of sperm (computer aided analysis parameters of semen such as the speed of sperm), and morphology of sperm (acrosome and membrane integrity).

Frozen-thawed semen with a higher sperm motility, viability, acrosome integrity and membrane integrity (P <0.05) was consistently higher in the acrosome reaction and sperm penetration test. Three bulls showed the highest, four bulls secondary display, and the remaining three cows lowest showing for all sperm parameters and SPA. seminal plasma proteome maps of high-quality and low-quality Bali cattle were also established. seminal plasma of both high quality and low quality bulls Bali was subjected to two-dimensional SDS-PAGE with isoelectric points ranging from 3 to 10 and a molecular weight of 10-250 kDa. Approximately 116 points detected by Blue Silver stains and spots, 29 have been selected and identified by MALDI-TOF / TOF-MS / MS.

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 652.5

Chiller for Electrophoresis systems

CSLCHILLER EACH
EUR 6793.2

Linear Polyacrylamide (5mg/ml)

S143 1 ml
EUR 48

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs

ELE2300 EACH
EUR 1602

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs

ELE2302 EACH
EUR 1681.2

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 712.8

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 742.5

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
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OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules

VS10WCES EACH
EUR 1434.85

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
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EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
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electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

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EV3610 ea
EUR 960

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EV3610-UK ea
EUR 960

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EV3610-US ea
EUR 960

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Accuris myGel Mini Electrophoresis Syst w/ Transf

ELE1111 EACH
EUR 854.4

electrophoresis power supply

EV245 ea
EUR 574.8

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
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Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

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EV245-UK ea
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ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
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CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
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LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

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LE1004 Set
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LE1005 Set
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LE1006 Set
EUR 1170

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

ES Electrophoresis Casting Tray, 7cm X 7cm

LE1011-A2 Ea
EUR 181.2

4 x Phosphate/SDS Electrophoresis Buffer

GR103033 1 L
EUR 116

Bovine Serum Albumin, 98% by electrophoresis

GE3699-10 10
EUR 28.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-100 100
EUR 142.4

Bovine Serum Albumin, 98% by electrophoresis

GE3699-25 25
EUR 50.6

Bovine Serum Albumin, 98% by electrophoresis

GE3699-250 250
EUR 284.8

Bovine Serum Albumin, 98% by electrophoresis

GE3699-500 500
EUR 506.2

ES Electrophoresis Casting Tray, 7cm X 14cm

LE1011-A1 Ea
EUR 198

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
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EDK613 1 UNIT
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EDK614 1 UNIT
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10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
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CometAssay Electrophoresis Starter Kit plus EU Power Supply

4250-050-ESK-PS2 1 Kit
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4250-050-ESK-PS3 1 Kit
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20-abx180004
  • EUR 393.60
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  • 100 mg
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Electro blot Mini system 10x10cm

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Glass tubes 24/150mm for Electrothermal STEM Integrity 10 system

HEA5636 PK10
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Stirrer bars 10/6mm for Electrothermal STEM Integrity 10 system

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PTFE cap with valve and sensor inlet for Electrothermal STEM Integrity 10 system

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Intrusive IT probe in stainless steel for Electrothermal STEM Integrity 10 system

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Axygen Gel Documentation System

GD-1000 EACH
EUR 5947.38

Gel Doc System EtBr Filter

ELE2500 EACH
EUR 7088.4

Nugenius Gel Docu System LED

ELE0028 EACH
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9K-006-0008 200preps, 200prep
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AXYGEN® GEL DOCUMENTATION SYSTEM BL

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Description: Lab Equipment; Axygen Branded EQ

Gel Filled Ph Electrode

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Casting Dams, For Enduro 7cm Gel System

LE1000CD-01 Ea
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LE1000CD-02 Ea
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LE1000CD-03 Ea
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Hach 5010T pH electrode Gel With Temperature Electrode

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10 Well Comb , 1mm Thick For Enduro 7cm Gel System

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10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

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16 Well Comb , 1mm Thick For Enduro 7cm Gel System

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20 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

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8 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

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25 Well Comb ,1mm Thick For Enduro 20cm Gel System

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8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
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12 Well Comb ,1mm Thick For Enduro 10cm Gel System

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12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

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16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

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16 Well Comb ,2mm Thick For Enduro 10cm Gel System

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25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
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8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
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10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
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10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
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15 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-03 Ea
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14 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-04 Ea
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16 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-05 Ea
EUR 157.2

20 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-11 Ea
EUR 157.2

16 Well Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-01 Ea
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12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
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16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 153.6

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-10 Ea
EUR 157.2

12 Well Mc Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-03 Ea
EUR 148.8

12 Well Mc Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-04 Ea
EUR 148.8

The majority of the proteins visualized in a two-dimensional map of the seminal plasma were identified. An important group of spots identified are represented binder sperm 1 (BSP1), clusterin, spermadhesin, tissue inhibitor of metalloproteinase 2 (TIMP-2), and phospholipase A2 (PLA2). Another protein found in high abundance including seminal ribonuclease, serum albumin, cationic trypsin, and peptide similar to the β2 -microglobulin. Thus, the reference maps Bali bull seminal plasma proteins have been produced for the first time and can be used to relate changes in the pattern of protein physiopathologic events that can affect the reproductive performance of Bali bull.

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