New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Current methods for identification and / or quantification of viral proteins of influenza virus and suffer virosome sample long analysis times, the coverage is limited protein and / or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to achieve a faster and improved characterization and quantification of viral proteins. sample preparation and gel buffer composition were investigated in order to achieve sufficient separation of proteins in a relatively short time.

Preparation of the total sample (reduction and deglycosylation) can be carried out efficiently within two hours. hydrodynamic injection, separation voltage, and temperature-optimized capillary in full factorial design. The latter method has been validated and shown good performance for hemagglutinin fragment 1 (HA1), hemagglutinin fragment 2 (HA2), the matrix protein (M) and nucleoprotein (NP). CGE method allows the identification of different viral strains based on their specific protein profiles. B / Brisbane attenuated virus and virosome samples can be analyzed in a single day.

The results of CGE (titer) is proportional to the radial immune-diffusion (SRID), but this method has the advantage of a much faster time to results. CGE analysis of A / Christchurch from upstream processes demonstrate the applicability of the method to samples of high complexity. CGE method can be used in the same analyte concentration range as the RP-HPLC method, but show the precision and accuracy that is better. Overall, the total analysis time for CGE method is much shorter, which allows the analysis of 100 samples within 4 days instead of 10 days for SRID.

Identification and comparative proteomic studies quail and duck egg white protein using 2-dimensional gel electrophoresis and matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry analysis.

A study of proteomics egg white protein of two species of large poultry, the quail (Coturnix Coturnix) and duck (Anas platyrhynchos), is done by comparison with those of the chicken (Gallus gallus) through 2-dimensional polyacrylamide gels (2-DE ) analysis. By using matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry (MALDI-TOF MS / MS), 29 where protein represents 10 types of protein and 17 where proteins appoint nine proteins have been identified in quail and duck egg white , each.

This report suggests a closer relationship between quail and chicken egg white proteome pattern, while a duck egg white protein distribution on the 2-DE map is much different. In the duck egg whites, some of the major proteins of the famous, such as ovomucoid, clusterin, extracellular fatty acid binding protein precursor (ex-FABP), and prostaglandin D2 synthase (PG D2 synthase), undetectable, while the two main proteins identified as “deleted malignant brain tumors 1 “protein (DMBT1) and vitellogenin-2 was found on a duck in the appropriate range on a map 2-DE gel.

This interspecies diversity may be related to egg white protein function in cellular defense or arrange / support embryo development to adapt to the environment that inhabit or reproduction demand for long-term evolution. The findings of this work will provide insight into the benefits involved in the application in the egg white protein from a variety of sources of eggs, which can lead to advantageous properties of the novel in the food industry or related to human health.

 New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.
New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Two-dimensional polyacrylamide gel electrophoresis Bali banteng (Bos javanicus) seminal plasma proteins and their association with semen quality.

This study evaluated the relationship between Bali banteng (Bos javanicus) protein seminal plasma and semen quality parameters are different. semen samples from 10 adult bulls Bali evaluated for semen parameters of conventional (motility general, viability, and normal morphology), sperm function (acrosome reaction, the rate of penetration of sperm, the index of penetration of sperm), the kinetics of sperm (computer aided analysis parameters of semen such as the speed of sperm), and morphology of sperm (acrosome and membrane integrity).

Frozen-thawed semen with a higher sperm motility, viability, acrosome integrity and membrane integrity (P <0.05) was consistently higher in the acrosome reaction and sperm penetration test. Three bulls showed the highest, four bulls secondary display, and the remaining three cows lowest showing for all sperm parameters and SPA. seminal plasma proteome maps of high-quality and low-quality Bali cattle were also established. seminal plasma of both high quality and low quality bulls Bali was subjected to two-dimensional SDS-PAGE with isoelectric points ranging from 3 to 10 and a molecular weight of 10-250 kDa. Approximately 116 points detected by Blue Silver stains and spots, 29 have been selected and identified by MALDI-TOF / TOF-MS / MS.

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7 each
EUR 892.8

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 3540.96

ComboMiniTM Horizontal Electrophoresis System (110V)

CBMN-7 each
EUR 500

ComboMiniTM Horizontal Electrophoresis System (220V)

CBMN-8 each
EUR 530

myGel Mini Electrophoresis System

E1101 1 each
EUR 636.38

myGel Mini Electrophoresis System

BM0286 System
EUR 741.6

miniONE Electrophoresis System - EACH

ELE3400 EACH
EUR 777.6

Cellulose Acetate Electrophoresis System

LA1027-1NO 1 unit
EUR 534.37
Description: Cellulose Acetate Electrophoresis System

WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

LA1070-1NO 1 unit
EUR 1258.71
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM

myGel Mini Electrophoresis System 230V (includes transformer)

E1101-E 1 PC
EUR 665.55

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 789.6

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3231 50T
EUR 100

High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system)

E1WP3232 100T
EUR 150

Biospin Polyacrylamide Gel DNA Extraction Kit

TRI-C15M1 100T
EUR 378

Biospin Polyacrylamide Gel DNA Extraction Kit

TRI-C15S1 50T
EUR 252

Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system)

E1WP326 50T
EUR 100

Agarose Gel Electrophoresis Kit

MB570-10PR 1 unit
EUR 38.47
Description: Agarose Gel Electrophoresis Kit

High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system)

E1WP324 50T
EUR 60

Chiller for Electrophoresis systems - EACH

CSLCHILLER EACH
EUR 7642.35

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator, 230V input

E1201-E 1 each
EUR 1058.9

runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH

ELE2300 EACH
EUR 1802.25

Polyacrylamide

P21450 250G
EUR 175

Agarose Gel Electrophoresis Kit (Teaching)

66602 15 expt. Kit
EUR 44.05
Description: Part E

runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH

ELE2302 EACH
EUR 1891.35

myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator

E1201 1 each
EUR 1001.7

Polyacrylamide (MW 10,000)

22581-100 100g
EUR 315
Description: 9003-05-8

HiPer® Agarose Gel Electrophoresis Teach

HTBM001-10PR 1 unit
EUR 29.56
Description: HiPer® Agarose Gel Electrophoresis Teach

myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100)

E1101-SK 1 each
EUR 727.06

Polyacrylamide (MW 5,000,000 - 6,000,000)

02806-250 250g
EUR 244
Description: 9003-05-8

Polyacrylamide (MW 5,000,000 - 6,000,000)

02806-50 50g
EUR 115
Description: 9003-05-8

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100)

E1101-SK-E 1 PC
EUR 757.35

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 513.6

WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell

HTES1013 set
EUR 602

WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell

HTES1014 set
EUR 448

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 148.8

Polyacrylamide (MW 5,000,000), 1% aq soln

21485-250 250g
EUR 210
Description: 9003-05-8

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 148.8

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-1 15 mL
EUR 17.3

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-2 25 mL
EUR 23.23

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-3 50 mL
EUR 35.42

Protein Antioxidant for SDS-PAGE Gel Electrophoresis

21440026-4 100 mL
EUR 62.99

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 898.8

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 898.8

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 898.8

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 960

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 960

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1456.8

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1456.8

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1456.8

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 684.96

Linear Polyacrylamide (5mg/ml)

S143 1 ml
EUR 48

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 686.4

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 686.4

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 960

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 960

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 960

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 574.8

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 574.8

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 686.4

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 686.4

OmniPage Mini Wide modular system inc tank capillary electrophoresis and electroblotting modules - EACH

VS10WCES EACH
EUR 1614.21

2xTaq PCR MasterMix for PAGE (with dye)(PAGE polyacrylamide gel dedicated 2x Taq Mix)

MBS355982-1mL 1mL
EUR 210

2xTaq PCR MasterMix for PAGE (with dye)(PAGE polyacrylamide gel dedicated 2x Taq Mix)

MBS355982-5mL 5mL
EUR 300

2xTaq PCR MasterMix for PAGE (with dye)(PAGE polyacrylamide gel dedicated 2x Taq Mix)

MBS355982-5x5mL 5x5mL
EUR 1050

Polyacrylamide 10% in water (MW 400,000 - 800,000)

19901-1 1Kg
EUR 486
Description: 9003-05-8

Polyacrylamide 10% in water (MW 400,000 - 800,000)

19901-250 250g
EUR 364
Description: 9003-05-8

Polyacrylamide (10% in H2O, Technical Grade)

P688150 10g
EUR 138
Description: 9003-05-8

Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH

ELE1111 EACH
EUR 961.2

Electrophoresis Buffer (10×)

E-BC-R331-100mL 100mL
EUR 15

Electrophoresis Buffer (10×)

E-BC-R331-200mL 200mL
EUR 20

Electrophoresis Buffer (10×)

E-BC-R331-500mL 500mL
EUR 25

Electrophoresis Buffer (10×)

E-BC-R331-each each Ask for price

Electrophoresis Buffer (10x)

MBS2557002-100mL 100mL
EUR 85

Electrophoresis Buffer (10x)

MBS2557002-200mL 200mL
EUR 90

Electrophoresis Buffer (10x)

MBS2557002-500mL 500mL
EUR 95

Electrophoresis Buffer (10x)

MBS2557002-5x500mL 5x500mL
EUR 380

TEMED, electrophoresis grade

RM10358-100G 1 unit
EUR 301.18
Description: TEMED, electrophoresis grade

Electrophoresis Power Supply

LA690-1NO 1 unit
EUR 432.84
Description: Electrophoresis Power Supply

electrophoresis power supply

EV245 ea
EUR 574.8

WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster)

HTES2001 set
EUR 322

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-1 10 mg
EUR 85.98

LPA (Linear Polyacrylamide) for DNA/RNA Precipitation

10510000-2 25 mg
EUR 140.95

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-005mL 0.05mL
EUR 195

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-01mL 0.1mL
EUR 255

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-5x005mL 5x0.05mL
EUR 820

SUPER Green II RNA gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579833-5x01mL 5x0.1mL
EUR 1100

Glycine for electrophoresis, 99.5%

52574 100 Gms
EUR 9.58
Description: Part A

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 410.4

10X MOPS Electrophoresis Buffer

ML050-500ML 1 unit
EUR 32.7
Description: 10X MOPS Electrophoresis Buffer

5 x SDS Electrophoresis Buffer

GR103039 1 L
EUR 107

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 578.4

SDS-PAGE Electrophoresis Buffer

MBS355536-1L 1L
EUR 165

SDS-PAGE Electrophoresis Buffer

MBS355536-5x1L 5x1L
EUR 480

SDS-PAGE Electrophoresis Buffer

MBS355542-10x1L 10x1L
EUR 250

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 750.6

Electrophoresis Module for Eco-Mini, for 2 gels

AJ846-017-175 each
EUR 604.95

Electrophoresis Module for Eco-Maxi, for 2 gels

AJ846-017-475 each
EUR 997.35

Starch (Smithies); for electrophoresis

GE6857 100g
EUR 253.58

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 370.8

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 217.2

electrophoresis power supply 120 vac

EV245-US ea
EUR 574.8

Aminoacetic acid, ?For electrophoresis

GRM2233-100G 1 unit
EUR 32.26
Description: Aminoacetic acid, ?For electrophoresis

Aminoacetic acid, ?For electrophoresis

GRM2233-500G 1 unit
EUR 144.96
Description: Aminoacetic acid, ?For electrophoresis

electrophoresis power supply uk plug

EV245-UK ea
EUR 574.8

ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels

LE1001 Set
EUR 651.6

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-005mL 0.05mL
EUR 180

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-01mL 0.1mL
EUR 230

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-05mL 0.5mL
EUR 695

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-5x005mL 5x0.05mL
EUR 760

SUPER Green I nucleic acid gel stain *10, 000× concentrate in DMSO* (Electrophoresis Grade)

MBS8579830-5x01mL 5x0.1mL
EUR 980

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3749.28

ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels

LE1002 Set
EUR 699.6

ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels

LE1003 Set
EUR 586.8

ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels

LE1004 Set
EUR 886.8

ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels

LE1005 Set
EUR 1081.2

ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels

LE1006 Set
EUR 1170

Agarose, medium EEO ?For electrophoresis

RM6249-100G 1 unit
EUR 119.06
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-10G 1 unit
EUR 19.63
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-1KG 1 unit
EUR 1089.43
Description: Agarose, medium EEO ?For electrophoresis

Agarose, medium EEO ?For electrophoresis

RM6249-25G 1 unit
EUR 36.87
Description: Agarose, medium EEO ?For electrophoresis

Alkaline Hemoglobin Electrophoresis Kit

MBP001-112RUNS 1 unit
EUR 118.87
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP001-224RUNS 1 unit
EUR 214
Description: Alkaline Hemoglobin Electrophoresis Kit

Alkaline Hemoglobin Electrophoresis Kit

MBP008-224RUNS 1 unit
EUR 208.42
Description: Alkaline Hemoglobin Electrophoresis Kit

Acrylamide, suitable for electrophoresis

GK5651 100g
EUR 21.8

Acrylamide, suitable for electrophoresis

GK5651-100 100
EUR 23.8

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 64.8

Alkaline Paper Electrophoresis Kit (Cell

MBP009-20RUNS 1 unit
EUR 59.3
Description: Alkaline Paper Electrophoresis Kit (Cell

Electrophoresis Power Supply (2 termina

LA842-1NO 1 unit
EUR 66.05
Description: Electrophoresis Power Supply (2 termina

WSHT-Basic Electrophoresis Power Supply

HTES1001 set
EUR 557.2

Electrophoresis baffle for Life mini tank

HTDB03 pcs
EUR 5.6

The majority of the proteins visualized in a two-dimensional map of the seminal plasma were identified. An important group of spots identified are represented binder sperm 1 (BSP1), clusterin, spermadhesin, tissue inhibitor of metalloproteinase 2 (TIMP-2), and phospholipase A2 (PLA2). Another protein found in high abundance including seminal ribonuclease, serum albumin, cationic trypsin, and peptide similar to the β2 -microglobulin. Thus, the reference maps Bali bull seminal plasma proteins have been produced for the first time and can be used to relate changes in the pattern of protein physiopathologic events that can affect the reproductive performance of Bali bull.

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