New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Current methods for identification and / or quantification of viral proteins of influenza virus and suffer virosome sample long analysis times, the coverage is limited protein and / or low accuracy and precision. We studied and optimized capillary gel electrophoresis (CGE) in order to achieve a faster and improved characterization and quantification of viral proteins. sample preparation and gel buffer composition were investigated in order to achieve sufficient separation of proteins in a relatively short time.

Preparation of the total sample (reduction and deglycosylation) can be carried out efficiently within two hours. hydrodynamic injection, separation voltage, and temperature-optimized capillary in full factorial design. The latter method has been validated and shown good performance for hemagglutinin fragment 1 (HA1), hemagglutinin fragment 2 (HA2), the matrix protein (M) and nucleoprotein (NP). CGE method allows the identification of different viral strains based on their specific protein profiles. B / Brisbane attenuated virus and virosome samples can be analyzed in a single day.

The results of CGE (titer) is proportional to the radial immune-diffusion (SRID), but this method has the advantage of a much faster time to results. CGE analysis of A / Christchurch from upstream processes demonstrate the applicability of the method to samples of high complexity. CGE method can be used in the same analyte concentration range as the RP-HPLC method, but show the precision and accuracy that is better. Overall, the total analysis time for CGE method is much shorter, which allows the analysis of 100 samples within 4 days instead of 10 days for SRID.

Identification and comparative proteomic studies quail and duck egg white protein using 2-dimensional gel electrophoresis and matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry analysis.

A study of proteomics egg white protein of two species of large poultry, the quail (Coturnix Coturnix) and duck (Anas platyrhynchos), is done by comparison with those of the chicken (Gallus gallus) through 2-dimensional polyacrylamide gels (2-DE ) analysis. By using matrix-assisted laser desorption / ionization time-of-flight tandem mass spectrometry (MALDI-TOF MS / MS), 29 where protein represents 10 types of protein and 17 where proteins appoint nine proteins have been identified in quail and duck egg white , each.

This report suggests a closer relationship between quail and chicken egg white proteome pattern, while a duck egg white protein distribution on the 2-DE map is much different. In the duck egg whites, some of the major proteins of the famous, such as ovomucoid, clusterin, extracellular fatty acid binding protein precursor (ex-FABP), and prostaglandin D2 synthase (PG D2 synthase), undetectable, while the two main proteins identified as “deleted malignant brain tumors 1 “protein (DMBT1) and vitellogenin-2 was found on a duck in the appropriate range on a map 2-DE gel.

This interspecies diversity may be related to egg white protein function in cellular defense or arrange / support embryo development to adapt to the environment that inhabit or reproduction demand for long-term evolution. The findings of this work will provide insight into the benefits involved in the application in the egg white protein from a variety of sources of eggs, which can lead to advantageous properties of the novel in the food industry or related to human health.

 New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.
New capillary gel electrophoresis method for fast and accurate identification and quantification of multiple viral proteins in influenza vaccines.

Two-dimensional polyacrylamide gel electrophoresis Bali banteng (Bos javanicus) seminal plasma proteins and their association with semen quality.

This study evaluated the relationship between Bali banteng (Bos javanicus) protein seminal plasma and semen quality parameters are different. semen samples from 10 adult bulls Bali evaluated for semen parameters of conventional (motility general, viability, and normal morphology), sperm function (acrosome reaction, the rate of penetration of sperm, the index of penetration of sperm), the kinetics of sperm (computer aided analysis parameters of semen such as the speed of sperm), and morphology of sperm (acrosome and membrane integrity).

Frozen-thawed semen with a higher sperm motility, viability, acrosome integrity and membrane integrity (P <0.05) was consistently higher in the acrosome reaction and sperm penetration test. Three bulls showed the highest, four bulls secondary display, and the remaining three cows lowest showing for all sperm parameters and SPA. seminal plasma proteome maps of high-quality and low-quality Bali cattle were also established. seminal plasma of both high quality and low quality bulls Bali was subjected to two-dimensional SDS-PAGE with isoelectric points ranging from 3 to 10 and a molecular weight of 10-250 kDa. Approximately 116 points detected by Blue Silver stains and spots, 29 have been selected and identified by MALDI-TOF / TOF-MS / MS.

8 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-17 Ea
EUR 131

myGel Mini Electrophoresis System

BM0286 System
EUR 618

CometAssay Single Cell Gel Electrophoresis Assay

4250-050-K 50 tests
EUR 428

CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 570.8

AXYGEN® GEL DOCUMENTATION SYSTEM

GD-1000 1/pk
EUR 4670
Description: Lab Equipment; Axygen Branded EQ

myGel Mini Electrophoresis System 230V (includes transformer)

BM0291 System
EUR 658

ES 6 Tooth Gel Comb For ES Electrophoresis

LE1011-A4 Ea
EUR 124

ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380

ACT-CBMN-7
EUR 744

Linear Polyacrylamide (5mg/ml)

S143 1 ml
EUR 40

AXYGEN® GEL DOCUMENTATION SYSTEM BL

GDBL-1000 1/pk
EUR 7632
Description: Lab Equipment; Axygen Branded EQ

ES 8/10 Double Gel Comb For ES Electrophoresis

LE1011-A3 Ea
EUR 124

Casting Dams, For Enduro 7cm Gel System

LE1000CD-01 Ea
EUR 126

Casting Dams, For Enduro 10cm Gel System

LE1000CD-02 Ea
EUR 138

Casting Dams, For Enduro 15cm Gel System

LE1000CD-03 Ea
EUR 165

Casting Dams, For Enduro 20cm Gel System

LE1000CD-04 Ea
EUR 192

Gel and PCR Extraction System (100 preps)

9K-006-0007 100preps
EUR 150.05
  • Product category: Molecular Biology Kits/DNA - Extraction/Gel + PCR Extraction

Gel and PCR Extraction System (200 Preps)

9K-006-0008 200preps, 200prep
EUR 219.65
  • Product category: Molecular Biology Kits/DNA - Extraction/Gel + PCR Extraction

myGel Mini Electrophoresis System Starter Kit (Includes E1101-E

E1101-SK-E 1 PC
EUR 535.53
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

17-Hydroxyprogesterone (17-OHP) ELISA Kit

DLR-17-OHP-Ge-48T 48T
EUR 469
  • Should the 17-Hydroxyprogesterone (17-OHP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

17-Hydroxyprogesterone (17-OHP) ELISA Kit

DLR-17-OHP-Ge-96T 96T
EUR 608
  • Should the 17-Hydroxyprogesterone (17-OHP) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of 17-Hydroxyprogesterone (17-OHP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RD-17-OHP-Ge-48Tests 48 Tests
EUR 467

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RD-17-OHP-Ge-96Tests 96 Tests
EUR 646

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RDR-17-OHP-Ge-48Tests 48 Tests
EUR 488

General 17-Hydroxyprogesterone (17-OHP) ELISA Kit

RDR-17-OHP-Ge-96Tests 96 Tests
EUR 676

electrophoresis power supply

EV245 ea
EUR 479

12 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-03 Ea
EUR 128

12 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-04 Ea
EUR 128

12 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-05 Ea
EUR 128

16 Well Comb ,0.75mm Thick For Enduro 10cm Gel System

LE1000C10-06 Ea
EUR 128

16 Well Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-07 Ea
EUR 128

16 Well Comb ,2mm Thick For Enduro 10cm Gel System

LE1000C10-08 Ea
EUR 128

25 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-11 Ea
EUR 128

8 Well Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-12 Ea
EUR 128

10 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-01 Ea
EUR 131

10 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-02 Ea
EUR 131

15 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-03 Ea
EUR 131

14 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-04 Ea
EUR 131

16 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-05 Ea
EUR 131

20 Well Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-10 Ea
EUR 131

20 Well Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-11 Ea
EUR 131

20 Well Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-12 Ea
EUR 131

20 Well Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-13 Ea
EUR 131

16 Well Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-01 Ea
EUR 138

25 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-05 Ea
EUR 138

30 Well Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-06 Ea
EUR 138

50 Well Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-10 Ea
EUR 138

10 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-01 Ea
EUR 124

10 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-02 Ea
EUR 124

16 Well Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-05 Ea
EUR 124

16 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-06 Ea
EUR 124

5 Well Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-07 Ea
EUR 124

8 Well Comb , 0.75mm Thick For Enduro 7cm Gel System

LE1000C7-08 Ea
EUR 124

8 Well Comb ,1mm Thick For Enduro 7cm Gel System

LE1000C7-09 Ea
EUR 124

GEL LOADING TIPS,10UL,FLAT,0.17MM OD,NONSTERILE,RACK,200/400

TGL-10FT-17-R 200/pk
EUR 141
Description: Non Filter Tips; Pipette Tips - Axygen

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 185

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1587

10 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-01 Ea
EUR 128

10 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-02 Ea
EUR 128

20 Well Mc Comb ,1mm Thick For Enduro 10cm Gel System

LE1000C10-09 Ea
EUR 128

20 Well Mc Comb ,1.5mm Thick For Enduro 10cm Gel System

LE1000C10-10 Ea
EUR 128

16 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-06 Ea
EUR 131

16 Well Mc Comb ,2mm Thick For Enduro 15cm Gel System

LE1000C15-07 Ea
EUR 131

18 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-08 Ea
EUR 131

18 Well Mc Comb ,1.5mm Thick For Enduro 15cm Gel System

LE1000C15-09 Ea
EUR 131

28 Well Mc Comb ,0.75mm Thick For Enduro 15cm Gel System

LE1000C15-14 Ea
EUR 131

28 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-15 Ea
EUR 131

30 Well Mc Comb ,1mm Thick For Enduro 15cm Gel System

LE1000C15-16 Ea
EUR 131

20 Well Mc Comb ,0.75mm Thick For Enduro 20cm Gel System

LE1000C20-02 Ea
EUR 138

20 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-03 Ea
EUR 138

20 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-04 Ea
EUR 138

40 Well Mc Comb ,1mm Thick For Enduro 20cm Gel System

LE1000C20-07 Ea
EUR 138

40 Well Mc Comb ,1.5mm Thick For Enduro 20cm Gel System

LE1000C20-08 Ea
EUR 138

40 Well Mc Comb ,2mm Thick For Enduro 20cm Gel System

LE1000C20-09 Ea
EUR 138

12 Well Mc Comb , 1mm Thick For Enduro 7cm Gel System

LE1000C7-03 Ea
EUR 124

12 Well Mc Comb , 1.5mm Thick For Enduro 7cm Gel System

LE1000C7-04 Ea
EUR 124

Gel-Bright LED Gel Illuminator

E90003 1EA
EUR 773
Description: Minimum order quantity: 1 unit of 1EA

FT3 (Free triiodothyronine) ELISA test

17 96T/Box Ask for price
  • Area of application: Hormone testing
Description: ELISA based test for quantitative detection of FT3 (Free triiodothyronine)

Acrylamide, suitable for electrophoresis

GK5651-100G 100 g
EUR 54

Starch (Smithies), for electrophoresis

GE6857-1KG 1 kg
EUR 309

Starch (Smithies), for electrophoresis

GE6857-500G 500 g
EUR 181

CometAssay Electrophoresis Starter Kit

4250-050-ESK 1 Kit
EUR 3124.4

Agarose for DNA electrophoresis

16-0027-500 500 g
EUR 342

ES Horizontal Electrophoresis Set

LE1011 Set
EUR 482

6X6 well Electrophoresis Apparatus

EDK626 1 UNIT
EUR 625.5
  • Product category: Benchtop Equipment/Gel Electrophoresis/6x6-well Systems

AXYGEN® GEL DOCUMENTATION SYSTEM BLUE LIGHT CONVERSION SCREEN FOR GD-1000

GD-BLCS 1/pk
EUR 700
Description: Lab Equipment; Axygen Branded EQ

Reproductive Tissue Dissociation System 17 (Sertoli), Mouse and Rat

4-20426 ea Ask for price

Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 175

Gel Tray

2394250 4unit
EUR 270
Description: I ncl udi ng pl at es

Gel Tray

2398194 2unit
EUR 354

TC Gel

CP048-005 500 g
EUR 232

TC Gel

CP048-010 1 Kg
EUR 347

Gel Cutter

KS071012-11 12
EUR 89
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Gel Filter

KS071012-13 12
EUR 115
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

electrophoresis ps 400v, 500ma, 50w

EV1450 ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-UK ea
EUR 479

electrophoresis ps 400v, 500ma, 50w

EV1450-US ea
EUR 479

electrophoresis ps 1500v, 300ma, 150w

EV2230 ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-UK ea
EUR 749

electrophoresis ps 1500v, 300ma, 150w

EV2230-US ea
EUR 749

electrophoresis ps 300v, 1000ma, 150w

EV2310 ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-UK ea
EUR 572

electrophoresis ps 300v, 1000ma, 150w

EV2310-US ea
EUR 572

electrophoresis ps 3000v, 150ma, 150w

EV2320 ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-UK ea
EUR 749

electrophoresis ps 3000v, 150ma, 150w

EV2320-US ea
EUR 749

electrophoresis power supply uk plug

EV245-UK ea
EUR 479

electrophoresis power supply 120 vac

EV245-US ea
EUR 479

electrophoresis ps 600v, 500ma, 150w

EV2650 ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-UK ea
EUR 572

electrophoresis ps 600v, 500ma, 150w

EV2650-US ea
EUR 572

electrophoresis ps 300v, 2000ma, 300w

EV3020 ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-UK ea
EUR 800

electrophoresis ps 300v, 2000ma, 300w

EV3020-US ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150 ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-UK ea
EUR 800

electrophoresis ps 1200v, 500ma, 300w

EV3150-US ea
EUR 800

electrophoresis ps 3000v, 300ma, 300w

EV3330 ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-UK ea
EUR 1214

electrophoresis ps 3000v, 300ma, 300w

EV3330-US ea
EUR 1214

electrophoresis ps 600v, 1000ma, 300w

EV3610 ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-UK ea
EUR 800

electrophoresis ps 600v, 1000ma, 300w

EV3610-US ea
EUR 800

electrophoresis ps 6000v, 150ma, 300w

EV3620 ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-UK ea
EUR 1214

electrophoresis ps 6000v, 150ma, 300w

EV3620-US ea
EUR 1214

Ketanserin (Vulketan Gel)

E1KS2232 50mg
EUR 434

AGAR, HIGH GEL

A01-102IHG-10kg 10 kg
EUR 2219

AGAR, HIGH GEL

A01-102IHG-2Kg 2 Kg
EUR 521

AGAR, HIGH GEL

A01-102IHG-500g 500 g
EUR 178

Gel tray L

2322112 5unit
EUR 257
Description: 2pcs/1set

Gel tray S

2322117 5unit
EUR 257
Description: 2pcs/1set

Gel Breaker Tubes

KS071012-12 12
EUR 89
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

ViSafe RED Gel

S430 500 µl
EUR 146

ViSafe RED Gel

S430L 5x500 µl
EUR 557

ViSafe Green Gel

S435 500 µl
EUR 146

ViSafe Green Gel

S435L 5x500 µl
EUR 557

Aluminium Phosphate Gel

VAdv-Ly0001 250 mL
EUR 1386
Description: Aluminium Phosphate Gel 2%, Aluminum salt Vaccine adjuvant.

Aluminium Hydroxide Gel

VAdv-Ly0003 250 mL
EUR 1386
Description: Aluminium Hydroxide Gel 2%, Aluminum salt Vaccine adjuvant.

Electrophoresis Apparatus - 7x10cm (6-10-well)

EDK611 1 UNIT
EUR 319.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/6-10-well Systems

Electrophoresis Apparatus - 7x14cm (6-10-well)

EDK613 1 UNIT
EUR 406.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/6-10-well Systems

10-well protein Vertical Electrophoresis Apparatus

EDK692 1 UNIT
EUR 406.26
  • Product category: Benchtop Equipment/Gel Electrophoresis/10-well Systems

AXYGEN® GEL DOCUMENTATION SYSTEM WHITE LIGHT CONVERSION SCREEN FOR GD-1000 AND GDBL-1000

GD-WLCS 1/pk
EUR 356
Description: Lab Equipment; Axygen Branded EQ

ProtoBlock System

NAT1004 1KIT
EUR 102

The majority of the proteins visualized in a two-dimensional map of the seminal plasma were identified. An important group of spots identified are represented binder sperm 1 (BSP1), clusterin, spermadhesin, tissue inhibitor of metalloproteinase 2 (TIMP-2), and phospholipase A2 (PLA2). Another protein found in high abundance including seminal ribonuclease, serum albumin, cationic trypsin, and peptide similar to the β2 -microglobulin. Thus, the reference maps Bali bull seminal plasma proteins have been produced for the first time and can be used to relate changes in the pattern of protein physiopathologic events that can affect the reproductive performance of Bali bull.

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