Proteins must maintain proper folding conformation and post-translational modifications express right (PTM) demonstrate appropriate biological activity. However, assessing protein folding and PTM difficult for routine polyacrylamide gel electrophoresis (PAGE) methods shortage separation resolution needed to identify variants of a single protein. In addition, standard PAGE denatures proteins prior to analysis of blocking determination PTM state or folding. To overcome this limitation, thermal gel electrophoresis microfluidic platform developed to provide high sensitivity, high-resolution analysis of the original protein variant.
A thermally reversible gel used as a temporary separation matrix in the solid state (30 ° C). gel provided hot enough resolution separation is to identify three different variants of fluorescently labeled proteins. To increase the sensitivity of detection, analyte preconcentration done in parallel with the separation. continuous analyte enrichment given the limit of detection of 500 fg protein (250 pM) while the resolution of basic simultaneous separation achieved between variants.
The effect of temperature on the thermal gel electrophoresis is also marked. Results depend on the unique temperature illustrates how the performance of the method can be tuned through thermal dimension. In the end, high-sensitivity detection and separation of resolution provided by the thermally activated gel electrophoresis rapid screening of the original protein-protein interactions variants.Protein, including oligomerization, is involved in the regulation of many cellular processes.
Unfortunately, many proteins are expressed at very low levels in vivo, making it challenging to observe oligomerization with size-exclusion chromatography, also known as gel filtration. In this protocol, we present the steps to perform the original blue polyacrylamide gel electrophoresis (BN-PAGE), a method for protein oligomer detailed studies on the plant. This article describes sample preparation of proteins from transgenic Arabidopsis thaliana and run the BN-PAGE gel followed by western blotting or otherwise direct, two-dimensional sodium dodecyl sulfide-polyacrylamide gel electrophoresis (2D SDS-PAGE). This protocol would be very helpful for new researchers performed experiments related to analyzing protein interactions stable including homo or hetero-oligomerization in plants. © 2020 The Authors.
Specific locations and Quantitative Analysis of N-glycan heterogeneity of Isomer Charge of Anti-VEGF Recombinant Protein Fusion by high resolution two-dimensional Gel Electrophoresis and Mass Spectrometry.
glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous. Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach.
Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges. Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of acid gel.

EFFECT OF TEMPERATURE IN sodium dodecyl sulphate Capillary gel electrophoresis (SDS-CGE) PROTEIN therapy.
Temperature dependent migration of standard molecular weight protein size and studied several biotherapeutic proteins in sodium dodecyl sulphate capillary gel electrophoresis in the interval of 15oC to 60oC using borate cross-linked dextran sieving matrix. Arrhenius plots were generated to calculate the value of the activation energy of each for various solute molecules. SDS-CGE analysis of a mixture of biotherapeutic protein test revealed no correlation between the activation energy requirements of the different species and their molecular weight, stressed the importance of temperature optimization to get high-resolution separation between solute molecules of interest.
Agarose, low EEO |
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GE0001-500G | Glentham Life Sciences | 500 g | EUR 418.8 |
Agarose, low EEO |
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GE0001-5G | Glentham Life Sciences | 5 g | EUR 58.8 |
Agarose B, Low EEO |
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AB0014 | Bio Basic | 50g | EUR 99.67 |
Agarose LE Low EEO |
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AXY1000 | Scientific Laboratory Supplies | PK10 | EUR 2058.84 |
Agarose LE Low EEO |
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AXY1002 | Scientific Laboratory Supplies | PK10 | EUR 8391.54 |
Agar, low gel strength |
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GK2227-100G | Glentham Life Sciences | 100 g | EUR 60 |
Agar, low gel strength |
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GK2227-1KG | Glentham Life Sciences | 1 kg | EUR 170.4 |
Agar, low gel strength |
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GK2227-250G | Glentham Life Sciences | 250 g | EUR 79.2 |
Agar, low gel strength |
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GK2227-500G | Glentham Life Sciences | 500 g | EUR 112.8 |
Agar, high gel strength |
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GK9085-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Agar, high gel strength |
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GK9085-1KG | Glentham Life Sciences | 1 kg | EUR 208.8 |
Agar, high gel strength |
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GK9085-250G | Glentham Life Sciences | 250 g | EUR 93.6 |
Agar, high gel strength |
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GK9085-500G | Glentham Life Sciences | 500 g | EUR 134.4 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
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GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
Agarose, Low melting gel |
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AB0015 | Bio Basic | 10g | EUR 96.54 |
Agar, low gel strength, suitable for microbiology |
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GC6234-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Agar, low gel strength, suitable for microbiology |
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GC6234-1KG | Glentham Life Sciences | 1 kg | EUR 184.8 |
Agar, low gel strength, suitable for microbiology |
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GC6234-250G | Glentham Life Sciences | 250 g | EUR 84 |
Agar, low gel strength, suitable for microbiology |
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GC6234-500G | Glentham Life Sciences | 500 g | EUR 118.8 |
Agarose for DNA electrophoresis |
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16-0027-500 | Medicago | 500 g | EUR 410.4 |
Agarose, medium EEO |
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GE4750-100G | Glentham Life Sciences | 100 g | EUR 199.2 |
Agarose, medium EEO |
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GE4750-500G | Glentham Life Sciences | 500 g | EUR 514.8 |
AGAROSE 25G-125G -500G |
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A01-118-10kg | Alphabiosciences | 10 kg | Ask for price |
AGAROSE 25G-125G -500G |
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A01-118-2kg | Alphabiosciences | 2kg | Ask for price |
AGAROSE 25G-125G -500G |
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A01-118-500g | Alphabiosciences | 500 g | EUR 592.8 |
HF Antidote Gel 25g |
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HFG25 | Scientific Laboratory Supplies | EACH | EUR 21.66 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT |
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AGR-LE-100 | CORNING | 1/pk | EUR 201.6 |
Description: Bioscience Mol Bio; Agarose |
ES 6 Tooth Gel Comb For ES Electrophoresis |
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LE1011-A4 | GenDepot | Ea | EUR 148.8 |
Doxycycline HCl, 25g |
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R1059-25g | ACTGene | each | EUR 565.2 |
Gentamycin Sulfate, 25g |
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R1405-25g | ACTGene | each | EUR 228 |
Kanamycin Sulfate, 25g |
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R2538-25g | ACTGene | each | EUR 228 |
Carbenicillin Disodium, 25g |
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R4800-25g | ACTGene | each | EUR 379.2 |
5-FOA, 25g |
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R7039-25g | ACTGene | each | EUR 1155.6 |
CometAssay Single Cell Gel Electrophoresis Assay |
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4250-050-K | Biotechne | 50 tests | EUR 513.6 |
ES 8/10 Double Gel Comb For ES Electrophoresis |
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LE1011-A3 | GenDepot | Ea | EUR 148.8 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 500G/UNIT, 10 UNITS PER CASE |
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AGR-LE-5001 | CORNING | 1/pk | EUR 3669.6 |
Description: Bioscience Mol Bio; Agarose |
Agarose |
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20-abx186369 | Abbexa |
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AGAR, HIGH GEL |
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A01-102IHG-10kg | Alphabiosciences | 10 kg | EUR 2662.8 |
AGAR, HIGH GEL |
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A01-102IHG-2Kg | Alphabiosciences | 2 Kg | EUR 625.2 |
AGAR, HIGH GEL |
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A01-102IHG-500g | Alphabiosciences | 500 g | EUR 213.6 |
Hi-Bind? T-Gel-Agarose |
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M1313-1 | Biovision | each | EUR 157.2 |
Hi-Bind? T-Gel-Agarose |
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M1313-10 | Biovision | each | EUR 261.6 |
Hi-Bind? T-Gel-Agarose |
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M1313-100 | Biovision | each | EUR 1801.2 |
AGAROSE LE, LOW EEO, MOLECULAR BIOLOGY GRADE, 100G PER UNIT, 10 UNITS PER CASE |
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AGR-LE-1001 | CORNING | 1/pk | EUR 926.4 |
Description: Bioscience Mol Bio; Agarose |
Kimas high strength centrifuge tubes |
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Z514861-6EA | Scientific Laboratory Supplies | PK6 | EUR 93.02 |
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
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4251-050-K | Biotechne | 1 kit | EUR 684.96 |
HiQTM Low Melting Agarose |
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A0223-002 | GenDepot | 25g | EUR 343.2 |
HiQTM Low Melting Agarose |
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A0223-010 | GenDepot | 100g | EUR 699.6 |
Agarose LM Low Melting |
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AXY1004 | Scientific Laboratory Supplies | PK10 | EUR 10277.1 |
Agarose LM Low Melting |
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AXY1006 | Scientific Laboratory Supplies | PK10 | EUR 5669.22 |
Rat IgG Enriched | RA-25G |
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RA-25G | Immunology Consultants Laboratory | 20 mg | EUR 274 |
Description: Rat IgG Enriched | RA-25G | Immunology Consultants Laboratory Species Reactivity: Rat Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
Agarose Gel DNA Extraction and Purification |
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EP10010 | Neuromics | 100 Tests | EUR 356.4 |
Agarose II, Low Gelling Temperature |
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CH002 | ABM | 25 g | EUR 274.8 |
Agarose II, Low Gelling Temperature |
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CH003 | ABM | 100 g | EUR 525.6 |
Agarose GPG/LMP low melt |
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A2501-100000 | ApexBio | 100 g | EUR 510 |
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
Agarose GPG/LMP low melt |
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A2501-25000 | ApexBio | 25 g | EUR 184.8 |
Description: Agarose has a wide range of physical, chemical and thermal stability, because of its greatest gelling ability that it acquires wide applications in biotechnology [2], such as agarose gel electrophoresis, protein purification, solid culture media, motility assays. |
Agarose gel DNA extraction and purification kit |
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K1441-100 | Biovision | each | EUR 510 |
Acrylamide, suitable for electrophoresis |
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GK5651-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Starch (Smithies), for electrophoresis |
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GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 370.8 |
Starch (Smithies), for electrophoresis |
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GE6857-500G | Glentham Life Sciences | 500 g | EUR 217.2 |
Chiller for Electrophoresis systems |
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CSLCHILLER | Scientific Laboratory Supplies | EACH | EUR 6453.54 |
Electrode Filling Sol Low Ionic Strength Applications |
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EFSLIAPP | Scientific Laboratory Supplies | 100ML | EUR 80.94 |
Preactivated Cys-Link-Affinity Gel Agarose (for Cys-containing peptides/proteins) |
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110100-CG | Alpha Diagnostics | 5 ml | EUR 343.2 |
Preactivated N-Link-Affinity Gel Agarose (for NH2-containing peptides/proteins) |
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110200-NG | Alpha Diagnostics | 5 ml | EUR 343.2 |
Keyhole Limpet Hemocyanin (KLH)-Agarose affinity gel for removing KLH antibodies |
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KLH11-G | Alpha Diagnostics | 1 ml | EUR 270 |
E. Coli Proteins-Agarose affinity gel for removing E. coli antibodies |
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EC11-G | Alpha Diagnostics | 1 ml | EUR 343.2 |
HyAgarose? LM Agarose, Low Melting, 100g |
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R9012LM-100g | ACTGene | each | EUR 452.4 |
HyAgarose? LM Agarose, Low Melting, 10g |
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R9012LM-10g | ACTGene | each | EUR 120 |
HyAgarose? LM Agarose, Low Melting, 500g |
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R9012LM-500g | ACTGene | each | EUR 1821.6 |
HyAgarose? LM Agarose, Low Melting, 50g |
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R9012LM-50g | ACTGene | each | EUR 262.8 |
Apex Low Melt Agarose Ultra Pure |
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20-103 | Genesee Scientific | 25g/Unit | EUR 375 |
Apex Low Melt Agarose Ultra Pure |
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20-104 | Genesee Scientific | 100g/Unit | EUR 687 |
Apex Low Melt Sieve Agarose Ultra Pure |
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20-245 | Genesee Scientific | 100g/Unit | EUR 566 |
Keyhole Limpet Hemocyanin (KLH)-Agarose af is finity gel for removing KLH antibodies |
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KLH11-G-5 | Alpha Diagnostics | 5 ml | EUR 634.8 |
ToxOut™ High Capacity Endotoxin Removal Agarose |
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7942-5 | Biovision | each | Ask for price |
25G Riboflavin |
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NAT1058 | Scientific Laboratory Supplies | 25G | EUR 40.47 |
25G PPO |
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NAT1498 | Scientific Laboratory Supplies | 25G | EUR 59.28 |
25G POPOP |
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NAT1524 | Scientific Laboratory Supplies | 25G | EUR 137.94 |
Kimax Reuseable Glass Centrifuge tubes plain high strength 15ml |
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TUB2718 | Scientific Laboratory Supplies | PK6 | EUR 110.58 |
dam for 15cm gel |
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EHS3400-DAM | Consort | ea | EUR 189.6 |
dam for 20cm gel |
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EHS3500-DAM | Consort | ea | EUR 199.2 |
dam for 23.5cm gel |
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EHS3600-DAM | Consort | ea | EUR 199.2 |
G418 Disulfate (Low Endotoxin) |
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G048-25G | TOKU-E | 25 g | EUR 493.2 |
Hydroxyethyl cellulose, low viscosity |
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GC0004-25G | Glentham Life Sciences | 25 g | EUR 49.2 |
Hydroxyethyl cellulose, high viscosity |
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GC2093-25G | Glentham Life Sciences | 25 g | EUR 48 |
Cys-link agarose gel coupling buffer kit (5-10 peptides) |
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110400-CB | Alpha Diagnostics | 1 kit | EUR 270 |
Rabbit Anti-E. Coli Proteins IgG-Agarose affinity gel for removing E. coli proteins |
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EC12-AS | Alpha Diagnostics | 1 ml | EUR 634.8 |
Agarose LE, Ultra-Pure Molecular Biology Grade, 25 g |
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41028-25G | Biotium | 25G | EUR 130.8 |
Description: Minimum order quantity: 1 unit of 25G |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
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P9301-005 | GenDepot | 5ml | EUR 273.6 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
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P9301-010 | GenDepot | 10ml | EUR 416.4 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
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P9301-050 | GenDepot | 50ml | EUR 1628.4 |
Protein A-Agarose High-Binding 6% Highly Crosslinked |
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P9301-100 | GenDepot | 100ml | EUR 2895.6 |
Hydroxyethyl cellulose, very low viscosity |
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GC9711-25G | Glentham Life Sciences | 25 g | EUR 48 |
High/low temp limiter w alarm |
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INC2190 | Scientific Laboratory Supplies | EACH | EUR 445.74 |
AF110 High and Low Chlorine Kit |
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WAT2060 | Scientific Laboratory Supplies | EACH | EUR 417.24 |
electrophoresis power supply |
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EV245 | Consort | ea | EUR 574.8 |
CometAssay Electrophoresis System |
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4250-050-ES | Biotechne | 1 Kit | EUR 3540.96 |
miniONE Electrophoresis System |
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ELE3400 | Scientific Laboratory Supplies | EACH | EUR 624.72 |
25G AquaPor LE |
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NAT1020 | Scientific Laboratory Supplies | 25G | EUR 79.8 |
25G AquaPor ES |
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NAT1026 | Scientific Laboratory Supplies | 25G | EUR 102.6 |
25G AquaPor LM |
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NAT1030 | Scientific Laboratory Supplies | 25G | EUR 249.66 |
25G AquaPor HR |
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NAT1034 | Scientific Laboratory Supplies | 25G | EUR 192.66 |
25G DATD Ultrapure |
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NAT1044 | Scientific Laboratory Supplies | 25G | EUR 121.98 |
25G Ammonium Persulfate |
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NAT1062 | Scientific Laboratory Supplies | 25G | EUR 40.47 |
25G Alcian Blue |
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NAT1344 | Scientific Laboratory Supplies | 25G | EUR 152.76 |
25G Biebrich Scarlet |
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NAT1346 | Scientific Laboratory Supplies | 25G | EUR 96.9 |
25G Basic Fuchsin |
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NAT1350 | Scientific Laboratory Supplies | 25G | EUR 328.32 |
25G Methylene Blue |
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NAT1352 | Scientific Laboratory Supplies | 25G | EUR 144.78 |
25G Butyl PBD |
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NAT1510 | Scientific Laboratory Supplies | 25G | EUR 150.48 |
25G p-Terphenyl |
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NAT1520 | Scientific Laboratory Supplies | 25G | EUR 109.44 |
25G Bis-MSB |
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NAT1528 | Scientific Laboratory Supplies | 25G | EUR 185.82 |
25g Chloramphenicol Powder |
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61-239-RI | Scientific Laboratory Supplies | EACH | EUR 98.04 |
Flash Silica 25g |
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CHR5178 | Scientific Laboratory Supplies | PK10 | EUR 117.42 |
Electrode Polish 25g |
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CHL2024 | Scientific Laboratory Supplies | EACH | EUR 30.32 |
Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2 |
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G2008-050 | GenDepot | 500g | EUR 345.6 |
Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2 |
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G2008-100 | GenDepot | 1Kg | EUR 549.6 |
Gellan Gum, Phyto Plant Culture Grade, ≥ 80% transmittance,Gel strength > 800 g/cm2 |
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G2008-500 | GenDepot | 5Kg | EUR 2004 |
Low Level TISAB II Ionic Strength Adjustment Buffer Solution for Calibration of Ion Selective Electrodes |
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IS-BUF3-500 | Scientific Laboratory Supplies | 500ML | EUR 125.4 |
gel casting base for evs3100 |
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EVS3100-BASE | Consort | ea | EUR 361.2 |
gel casting base for evs3200 |
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EVS3200-BASE | Consort | ea | EUR 361.2 |
gel casting base for evs3300 |
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EVS3300-BASE | Consort | ea | EUR 361.2 |
Gel tray V (for Visirays) |
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2195934 | Atto | 2unit | EUR 356.4 |
Description: Opt i on |
Gel carrier for AE-6541 |
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2394140 | Atto | 10unit | EUR 303.6 |
Agarose |
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abx082006-100g | Abbexa | 100 g | EUR 292.8 |
Agarose |
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abx082007-100g | Abbexa | 100 g | EUR 309.6 |
Agarose |
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abx082008-100g | Abbexa | 100 g | EUR 276 |
Agarose |
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20-abx098147 | Abbexa |
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Agarose |
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G060-1 | ABM | 100 g | EUR 211.2 |
Agarose |
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G060-2 | ABM | 500 g | EUR 442.8 |
Agarose |
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Z5050007 | Biochain | 500 g | EUR 738 |
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
Agarose |
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Z5050013 | Biochain | 100 g | EUR 226.8 |
Description: None pays much attention to the agarose until your gel comes out all with waves and uneven thickness. Or even worse, the gel comes out all good only to start melting while you are running the electrophoresis wasting you precious samples. Save yourself some trouble and get our agarose which will provide you with excellent gel quality every time. |
pH/ATC Triode with epoxy body low maintenance gel |
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9107BNMD | Scientific Laboratory Supplies | EACH | EUR 265.62 |
Combination pH electrode with epoxy body low maintenance gel |
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9146BN | Scientific Laboratory Supplies | EACH | EUR 101.46 |
Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2 |
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G2007-050 | GenDepot | 500g | EUR 360 |
Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2 |
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G2007-100 | GenDepot | 1Kg | EUR 562.8 |
Gellan Gum, Pure, Plant Culture Grade, ≥ 80% transmittance,Gel strength 400-700 g/cm2 |
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G2007-500 | GenDepot | 5Kg | EUR 2101.2 |
Chitosan (5 cps), ultra low molecular weight |
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GP1318-25G | Glentham Life Sciences | 25 g | EUR 60 |
Chitosan (10 cps), very low molecular weight |
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GP5480-25G | Glentham Life Sciences | 25 g | EUR 60 |
Chitosan (100 - 300 cps), low molecular weight |
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GP7325-25G | Glentham Life Sciences | 25 g | EUR 60 |
Chitosan (30 - 100 cps), low molecular weight |
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GP8523-25G | Glentham Life Sciences | 25 g | EUR 60 |
Maximo-Gel juice fluorescent Protein-Gel Gel stain |
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310012 | GeneOn | 10 ml | EUR 222 |
Maximo-Gel juice fluorescent Protein-Gel Gel stain |
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310012-L | GeneOn | 10x10ml | EUR 1904.4 |
Chitosan (1000 - 2000 cps), high molecular weight |
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GP5053-25G | Glentham Life Sciences | 25 g | EUR 60 |
Gel-Bright LED Gel Illuminator |
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E90003 | Biotium | 1EA | EUR 927.6 |
Description: Minimum order quantity: 1 unit of 1EA |
CometAssay Electrophoresis Starter Kit |
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4250-050-ESK | Biotechne | 1 Kit | EUR 3749.28 |
myGel Mini Electrophoresis System |
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BM0286 | GenDepot | System | EUR 741.6 |
ES Horizontal Electrophoresis Set |
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LE1011 | GenDepot | Set | EUR 578.4 |
6X6 well Electrophoresis Apparatus |
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EDK626 | Bio Basic | 1 UNIT | EUR 750.6 |
myGel Mini Electrophoresis System |
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E1101 | Benchmark Scientific | 1 each | EUR 623.9 |
Gel Casting set for 10.5x6cm gels |
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E1101-CS1 | Benchmark Scientific | 1 PC | EUR 114.37 |
Gel Casting Stand for 5x6cm gels |
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E1101-CS2 | Benchmark Scientific | 1 PC | EUR 135.42 |
Gel Casting S for WSE-1710 |
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2322107 | Atto | 4unit | EUR 339.6 |
Description: Gel Tr ay (S) x4, Gel Cast i ng St and (S), S si zecomb (9wel l /5wel l ) x2 |
Gel Casting L for WSE-1710 |
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2322108 | Atto | 4unit | EUR 339.6 |
Description: Gel Tr ay (L ) x2, Gel Cast i ng St and (L ), S si zecomb (22wel l /12wel l ) x2 |
supporting mask for midi gel dryer |
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EDRY1100-MASK | Consort | ea | EUR 361.2 |
ssilicone rubber for midi gel dryer |
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EDRY1100-SILRUB | Consort | ea | EUR 108 |
supporting mask for maxi gel dryer |
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EDRY1200-MASK | Consort | ea | EUR 574.8 |
In contrast, protein molecular weight ladder sizes ranging from 10 kDa to 225 kDa, built of blocks of the same polypeptide without post-translational modifications and others, showed activation of predicted energy requirements. Electrophoretic mobility of SDS – protein complex found root function-6 reciprocal of the molecular weight (Mw-sixth), implying a cylindrical conformation. Modifications give us a better result in terms of yield protein, resolution, spot number, and intensity for 2-DE gel of D. hirsuta and two other liverworts, namely, marchantia paleacea and Plagiochasma appendiculatum. In addition, we selected at random points on 2-DE gels D. hirsuta and identified using mass spectrometry, which confirmed the application of this protocol to liverworts proteomics.