Proteins must maintain proper folding conformation and post-translational modifications express right (PTM) demonstrate appropriate biological activity. However, assessing protein folding and PTM difficult for routine polyacrylamide gel electrophoresis (PAGE) methods shortage separation resolution needed to identify variants of a single protein. In addition, standard PAGE denatures proteins prior to analysis of blocking determination PTM state or folding. To overcome this limitation, thermal gel electrophoresis microfluidic platform developed to provide high sensitivity, high-resolution analysis of the original protein variant.
A thermally reversible gel used as a temporary separation matrix in the solid state (30 ° C). gel provided hot enough resolution separation is to identify three different variants of fluorescently labeled proteins. To increase the sensitivity of detection, analyte preconcentration done in parallel with the separation. continuous analyte enrichment given the limit of detection of 500 fg protein (250 pM) while the resolution of basic simultaneous separation achieved between variants.
The effect of temperature on the thermal gel electrophoresis is also marked. Results depend on the unique temperature illustrates how the performance of the method can be tuned through thermal dimension. In the end, high-sensitivity detection and separation of resolution provided by the thermally activated gel electrophoresis rapid screening of the original protein-protein interactions variants.Protein, including oligomerization, is involved in the regulation of many cellular processes.
Unfortunately, many proteins are expressed at very low levels in vivo, making it challenging to observe oligomerization with size-exclusion chromatography, also known as gel filtration. In this protocol, we present the steps to perform the original blue polyacrylamide gel electrophoresis (BN-PAGE), a method for protein oligomer detailed studies on the plant. This article describes sample preparation of proteins from transgenic Arabidopsis thaliana and run the BN-PAGE gel followed by western blotting or otherwise direct, two-dimensional sodium dodecyl sulfide-polyacrylamide gel electrophoresis (2D SDS-PAGE). This protocol would be very helpful for new researchers performed experiments related to analyzing protein interactions stable including homo or hetero-oligomerization in plants. © 2020 The Authors.
Specific locations and Quantitative Analysis of N-glycan heterogeneity of Isomer Charge of Anti-VEGF Recombinant Protein Fusion by high resolution two-dimensional Gel Electrophoresis and Mass Spectrometry.
glycan modification request important concerns about quality control of biopharmaceutical production. Conbercept is a recombinant fusion protein glycosylation multi-drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in monomer, charge separation and characterization conbercept isomer pose a big challenge because of the heterogeneity enormous. Stability of batch-to-batch on the distribution charge isomers and the possible causal pattern requires the development of an effective analytical approach.
Here, moving the pH gradient (IPG) -based approaches the two-dimensional gel electrophoresis (2-DE) was first optimized to achieve high resolution, high separation and preparation costs reproduced isomers. Then, combined with a quantitative analysis of heterogeneity strategy-specific N-glycan based diagnostic MS2 ion (peptide + GlcNAc, ion Y1) of glycopeptides, integrated approach to heterogeneities quantitation of specific N-glycan isomers established between charges. Finally, the site-specific quantitation of N-glycoforms in each of the 2-DE resolved spots performed, and the results showed that sialylaion which tends to increase to a place situated in the area of acid gel.
EFFECT OF TEMPERATURE IN sodium dodecyl sulphate Capillary gel electrophoresis (SDS-CGE) PROTEIN therapy.
Temperature dependent migration of standard molecular weight protein size and studied several biotherapeutic proteins in sodium dodecyl sulphate capillary gel electrophoresis in the interval of 15oC to 60oC using borate cross-linked dextran sieving matrix. Arrhenius plots were generated to calculate the value of the activation energy of each for various solute molecules. SDS-CGE analysis of a mixture of biotherapeutic protein test revealed no correlation between the activation energy requirements of the different species and their molecular weight, stressed the importance of temperature optimization to get high-resolution separation between solute molecules of interest.
Agarose Gel Electrophoresis Kit |
|||
MB570-10PR | EWC Diagnostics | 1 unit | EUR 38.47 |
Description: Agarose Gel Electrophoresis Kit |
HiPer® Agarose Gel Electrophoresis Teach |
|||
HTBM001-10PR | EWC Diagnostics | 1 unit | EUR 29.56 |
Description: HiPer® Agarose Gel Electrophoresis Teach |
Agarose for DNA electrophoresis |
|||
16-0027-500 | Medicago | 500 g | EUR 410.4 |
ES 6 Tooth Gel Comb For ES Electrophoresis |
|||
LE1011-A4 | GenDepot | Ea | EUR 148.8 |
ES 8/10 Double Gel Comb For ES Electrophoresis |
|||
LE1011-A3 | GenDepot | Ea | EUR 148.8 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
|||
21440026-1 | Glycomatrix | 15 mL | EUR 17.3 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
|||
21440026-2 | Glycomatrix | 25 mL | EUR 23.23 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
|||
21440026-3 | Glycomatrix | 50 mL | EUR 35.42 |
Protein Antioxidant for SDS-PAGE Gel Electrophoresis |
|||
21440026-4 | Glycomatrix | 100 mL | EUR 62.99 |
CometAssay Single Cell Gel Electrophoresis Assay |
|||
4250-050-K | Biotechne | 50 tests | EUR 513.6 |
WSHT-mulitiPRO4 Precast Gel Electrophoresis Cell |
|||
HTES1013 | Shanghai WSHT Biotechnology | set | EUR 602 |
WSHT-mulitiPRO2 Precast Gel Electrophoresis Cell |
|||
HTES1014 | Shanghai WSHT Biotechnology | set | EUR 448 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
|||
E1WP3231 | EnoGene | 50T | EUR 100 |
High resolution protein SDS-PAGE electrophoresis pre mixed gel Kit (BIS Tris system) |
|||
E1WP3232 | EnoGene | 100T | EUR 150 |
Gel Electrophoresis System with Time Control, 220-240 V |
|||
Q-EP-2114 | ABM | 1 | EUR 1180 |
CometAssay Single Cell Gel Electrophoresis Assay-Silver |
|||
4251-050-K | Biotechne | 1 kit | EUR 684.96 |
High molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tris acetate system) |
|||
E1WP324 | EnoGene | 50T | EUR 60 |
Gel Electrophoresis System with Muticaster, 100-120 V Input |
|||
Q-EP-2034 | ABM | 1 | EUR 1715 |
Gel Electrophoresis System with Muticaster, 220-240 V Input |
|||
Q-EP-2056 | ABM | 1 | EUR 2125 |
Starch (Smithies), for electrophoresis |
|||
GE6857-1KG | Glentham Life Sciences | 1 kg | EUR 370.8 |
Starch (Smithies), for electrophoresis |
|||
GE6857-500G | Glentham Life Sciences | 500 g | EUR 217.2 |
Aminoacetic acid, ?For electrophoresis |
|||
GRM2233-100G | EWC Diagnostics | 1 unit | EUR 32.26 |
Description: Aminoacetic acid, ?For electrophoresis |
Aminoacetic acid, ?For electrophoresis |
|||
GRM2233-500G | EWC Diagnostics | 1 unit | EUR 144.96 |
Description: Aminoacetic acid, ?For electrophoresis |
Gel Electrophoresis System with Time Control, 100-120 V Input |
|||
Q-EP-2100 | ABM | 1 | EUR 1385 |
Acrylamide, suitable for electrophoresis |
|||
GK5651-100 | Glentham Life Sciences | 100 | EUR 23.8 |
Acrylamide, suitable for electrophoresis |
|||
GK5651-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Chiller for Electrophoresis systems - EACH |
|||
CSLCHILLER | Scientific Laboratory Supplies | EACH | EUR 7642.35 |
Low molecular weight protein SDS-PAGE electrophoresis pre mixed gel Kit (Tricine system) |
|||
E1WP326 | EnoGene | 50T | EUR 100 |
Electrophoresis baffle for Life mini tank |
|||
HTDB03 | Shanghai WSHT Biotechnology | pcs | EUR 5.6 |
High Molecular Weight Native Electrophoresis Protein Marker (66-669kDa) |
|||
MKPZ010-200mL | Shanghai WSHT Biotechnology | 200μL | EUR 72 |
electrophoresis ps 1500v, 300ma, 150w |
|||
EV2230 | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
|||
EV2230-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 1500v, 300ma, 150w |
|||
EV2230-US | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
|||
EV2320 | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
|||
EV2320-UK | Consort | ea | EUR 898.8 |
electrophoresis ps 3000v, 150ma, 150w |
|||
EV2320-US | Consort | ea | EUR 898.8 |
electrophoresis ps 1200v, 500ma, 300w |
|||
EV3150 | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
|||
EV3150-UK | Consort | ea | EUR 960 |
electrophoresis ps 1200v, 500ma, 300w |
|||
EV3150-US | Consort | ea | EUR 960 |
electrophoresis ps 3000v, 300ma, 300w |
|||
EV3330 | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
|||
EV3330-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 3000v, 300ma, 300w |
|||
EV3330-US | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
|||
EV3620 | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
|||
EV3620-UK | Consort | ea | EUR 1456.8 |
electrophoresis ps 6000v, 150ma, 300w |
|||
EV3620-US | Consort | ea | EUR 1456.8 |
Electrophoresis Buffer (10×) |
|||
E-BC-R331-100mL | Elabscience Biotech | 100mL | EUR 15 |
Electrophoresis Buffer (10×) |
|||
E-BC-R331-200mL | Elabscience Biotech | 200mL | EUR 20 |
Electrophoresis Buffer (10×) |
|||
E-BC-R331-500mL | Elabscience Biotech | 500mL | EUR 25 |
Electrophoresis Buffer (10×) |
|||
E-BC-R331-each | Elabscience Biotech | each | Ask for price |
Electrophoresis baffle for Life Technology Novex |
|||
HTDB02 | Shanghai WSHT Biotechnology | pcs | EUR 2.8 |
electrophoresis ps 400v, 500ma, 50w |
|||
EV1450 | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
|||
EV1450-UK | Consort | ea | EUR 574.8 |
electrophoresis ps 400v, 500ma, 50w |
|||
EV1450-US | Consort | ea | EUR 574.8 |
electrophoresis ps 300v, 1000ma, 150w |
|||
EV2310 | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
|||
EV2310-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 1000ma, 150w |
|||
EV2310-US | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
|||
EV2650 | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
|||
EV2650-UK | Consort | ea | EUR 686.4 |
electrophoresis ps 600v, 500ma, 150w |
|||
EV2650-US | Consort | ea | EUR 686.4 |
electrophoresis ps 300v, 2000ma, 300w |
|||
EV3020 | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
|||
EV3020-UK | Consort | ea | EUR 960 |
electrophoresis ps 300v, 2000ma, 300w |
|||
EV3020-US | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
|||
EV3610 | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
|||
EV3610-UK | Consort | ea | EUR 960 |
electrophoresis ps 600v, 1000ma, 300w |
|||
EV3610-US | Consort | ea | EUR 960 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
|||
20840057-1 | Glycomatrix | 500 mL | EUR 74.66 |
Tris-HEPES-SDS Buffer 20X for Electrophoresis |
|||
20840057-2 | Glycomatrix | 1 L | EUR 139.51 |
TEMED, electrophoresis grade |
|||
RM10358-100G | EWC Diagnostics | 1 unit | EUR 301.18 |
Description: TEMED, electrophoresis grade |
electrophoresis power supply |
|||
EV245 | Consort | ea | EUR 574.8 |
Electrophoresis Power Supply |
|||
LA690-1NO | EWC Diagnostics | 1 unit | EUR 432.84 |
Description: Electrophoresis Power Supply |
Electrophoresis baffle for Bio-Rad Mini-PROTEAN |
|||
HTDB01 | Shanghai WSHT Biotechnology | pcs | EUR 2.1 |
ComboMini? Electrophoresis System ? COMPLETE with ONE electrophoresis system + ONE gel maker set (4 combs, 1 large tray, 1 medium tray, 1 small tray, 1 gel casting stand) NEW (specify 220V or 110V) $380 |
|||
ACT-CBMN-7 | ACTGene | each | EUR 892.8 |
10X MOPS Electrophoresis Buffer |
|||
ML050-500ML | EWC Diagnostics | 1 unit | EUR 32.7 |
Description: 10X MOPS Electrophoresis Buffer |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell (without Gel Caster) |
|||
HTES2001 | Shanghai WSHT Biotechnology | set | EUR 322 |
5 x SDS Electrophoresis Buffer |
|||
GR103039 | Genorise Scientific | 1 L | EUR 107 |
CometAssay Electrophoresis System |
|||
4250-050-ES | Biotechne | 1 Kit | EUR 3540.96 |
myGel Mini Electrophoresis System |
|||
E1101 | Benchmark Scientific | 1 each | EUR 636.38 |
myGel Mini Electrophoresis System |
|||
BM0286 | GenDepot | System | EUR 741.6 |
ES Horizontal Electrophoresis Set |
|||
LE1011 | GenDepot | Set | EUR 578.4 |
6X6 well Electrophoresis Apparatus |
|||
EDK626 | Bio Basic | 1 UNIT | EUR 750.6 |
miniONE Electrophoresis System - EACH |
|||
ELE3400 | Scientific Laboratory Supplies | EACH | EUR 777.6 |
electrophoresis power supply 120 vac |
|||
EV245-US | Consort | ea | EUR 574.8 |
electrophoresis power supply uk plug |
|||
EV245-UK | Consort | ea | EUR 574.8 |
ENDURO 7.7 Horizontal Electrophoresis 7 x 7cm Gels |
|||
LE1001 | GenDepot | Set | EUR 651.6 |
CometAssay Electrophoresis Starter Kit |
|||
4250-050-ESK | Biotechne | 1 Kit | EUR 3749.28 |
ENDURO 7.10 Horizontal Electrophoresis 7 x 10cm Gels |
|||
LE1002 | GenDepot | Set | EUR 699.6 |
ENDURO 10.10 Horizontal Electrophoresis 10 x 10cm Gels |
|||
LE1003 | GenDepot | Set | EUR 586.8 |
ENDURO 15.10 Horizontal Electrophoresis 15 x 10cm Gels |
|||
LE1004 | GenDepot | Set | EUR 886.8 |
ENDURO 15.15 Horizontal Electrophoresis 15 x 15cm Gels |
|||
LE1005 | GenDepot | Set | EUR 1081.2 |
ENDURO 20.20 Horizontal Electrophoresis 20 x 20cm Gels |
|||
LE1006 | GenDepot | Set | EUR 1170 |
Alkaline Hemoglobin Electrophoresis Kit |
|||
MBP001-112RUNS | EWC Diagnostics | 1 unit | EUR 118.87 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
|||
MBP001-224RUNS | EWC Diagnostics | 1 unit | EUR 214 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Hemoglobin Electrophoresis Kit |
|||
MBP008-224RUNS | EWC Diagnostics | 1 unit | EUR 208.42 |
Description: Alkaline Hemoglobin Electrophoresis Kit |
Alkaline Paper Electrophoresis Kit (Cell |
|||
MBP009-20RUNS | EWC Diagnostics | 1 unit | EUR 59.3 |
Description: Alkaline Paper Electrophoresis Kit (Cell |
WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
|||
LA1070-1NO | EWC Diagnostics | 1 unit | EUR 1258.71 |
Description: WEE VERT PROTEIN ELECTROPHORESIS SYSTEM |
Electrophoresis Power Supply (2 termina |
|||
LA842-1NO | EWC Diagnostics | 1 unit | EUR 66.05 |
Description: Electrophoresis Power Supply (2 termina |
Cellulose Acetate Electrophoresis System |
|||
LA1027-1NO | EWC Diagnostics | 1 unit | EUR 534.37 |
Description: Cellulose Acetate Electrophoresis System |
WSHT-Basic Electrophoresis Power Supply |
|||
HTES1001 | Shanghai WSHT Biotechnology | set | EUR 557.2 |
ES Electrophoresis Casting Tray, 7cm X 7cm |
|||
LE1011-A2 | GenDepot | Ea | EUR 181.2 |
Bovine Serum Albumin, 98% by electrophoresis |
|||
GE3699-10 | Glentham Life Sciences | 10 | EUR 28.4 |
Bovine Serum Albumin, 98% by electrophoresis |
|||
GE3699-100 | Glentham Life Sciences | 100 | EUR 142.4 |
Bovine Serum Albumin, 98% by electrophoresis |
|||
GE3699-25 | Glentham Life Sciences | 25 | EUR 50.6 |
Bovine Serum Albumin, 98% by electrophoresis |
|||
GE3699-250 | Glentham Life Sciences | 250 | EUR 284.8 |
Bovine Serum Albumin, 98% by electrophoresis |
|||
GE3699-500 | Glentham Life Sciences | 500 | EUR 506.2 |
4 x Phosphate/SDS Electrophoresis Buffer |
|||
GR103033 | Genorise Scientific | 1 L | EUR 116 |
ES Electrophoresis Casting Tray, 7cm X 14cm |
|||
LE1011-A1 | GenDepot | Ea | EUR 198 |
Hi-Eco Mini Horizontal Electrophoresis K |
|||
LA851-1NO | EWC Diagnostics | 1 unit | EUR 205.56 |
Description: Hi-Eco Mini Horizontal Electrophoresis K |
Electrophoresis Apparatus - 7x10cm (6-10-well) |
|||
EDK611 | Bio Basic | 1 UNIT | EUR 383.11 |
Electrophoresis Apparatus - 7x14cm (6-10-well) |
|||
EDK613 | Bio Basic | 1 UNIT | EUR 487.51 |
WSHT-Universal Electrophoresis Power Supply |
|||
HTES1003 | Shanghai WSHT Biotechnology | set | EUR 784 |
ComboMiniTM Horizontal Electrophoresis System (110V) |
|||
CBMN-7 | Avans Biotechnology Corp | each | EUR 500 |
ComboMiniTM Horizontal Electrophoresis System (220V) |
|||
CBMN-8 | Avans Biotechnology Corp | each | EUR 530 |
WSHT-miniPRO4 Mini Vertical Electrophoresis Cell |
|||
HTES1009 | Shanghai WSHT Biotechnology | set | EUR 795.2 |
WSHT-miniPRO2 Mini Vertical Electrophoresis Cell |
|||
HTES1010 | Shanghai WSHT Biotechnology | set | EUR 516.6 |
WSHT-easyPRO4 Mini Vertical Electrophoresis Cell |
|||
HTES1011 | Shanghai WSHT Biotechnology | set | EUR 837.2 |
WSHT-easyPRO2 Mini Vertical Electrophoresis Cell |
|||
HTES1012 | Shanghai WSHT Biotechnology | set | EUR 544.6 |
Electrophoresis Apparatus - 7x7cm Dual (2x 6-10-well) |
|||
EDK614 | Bio Basic | 1 UNIT | EUR 520.92 |
WSHT-miniDNA Mini Horizontal Electrophoresis Cell |
|||
HTES1008 | Shanghai WSHT Biotechnology | set | EUR 193.2 |
10-well protein Vertical Electrophoresis Apparatus |
|||
EDK692 | Bio Basic | 1 UNIT | EUR 487.51 |
Agar, High Gel Strength |
|||
40100064-1 | Glycomatrix | 500 g | EUR 84.23 |
Agar, High Gel Strength |
|||
40100064-2 | Glycomatrix | 1 kg | EUR 135.61 |
Agar, High Gel Strength |
|||
40100064-3 | Glycomatrix | 2 kg | EUR 256.75 |
Agar, High Gel Strength |
|||
40100064-4 | Glycomatrix | 5 kg | EUR 557.75 |
Agar, high gel strength |
|||
GK9085-1 | Glentham Life Sciences | 1 | EUR 142.4 |
Agar, high gel strength |
|||
GK9085-100 | Glentham Life Sciences | 100 | EUR 23.8 |
Agar, high gel strength |
|||
GK9085-100G | Glentham Life Sciences | 100 g | EUR 64.8 |
Agar, high gel strength |
|||
GK9085-1KG | Glentham Life Sciences | 1 kg | EUR 208.8 |
Agar, high gel strength |
|||
GK9085-250 | Glentham Life Sciences | 250 | EUR 47.4 |
Agar, high gel strength |
|||
GK9085-250G | Glentham Life Sciences | 250 g | EUR 93.6 |
Agar, high gel strength |
|||
GK9085-500 | Glentham Life Sciences | 500 | EUR 81.5 |
Agar, high gel strength |
|||
GK9085-500G | Glentham Life Sciences | 500 g | EUR 134.4 |
Accuris myGel Mini Electrophoresis Syst w/ Transf - EACH |
|||
ELE1111 | Scientific Laboratory Supplies | EACH | EUR 961.2 |
WSHT-Multi-Functional Electrophoresis Power Supply |
|||
HTES1004 | Shanghai WSHT Biotechnology | set | EUR 896 |
SUPER-Green-II-RNA-gel-stain-*10,000×-concentrate-in-DMSO*--(Electrophoresis-Grade) |
|||
E37F007 | EnoGene | 100-μL | EUR 226.67 |
Description: Reagents-for-ssDNA-or-RNA-electrophoresis |
WSHT-SCZ2+ Mini-Protean Vertical Electrophoresis Cell |
|||
HTES2001-P | Shanghai WSHT Biotechnology | set | EUR 459.2 |
WSHT-liteDNA Universal Horizontal Electrophoresis Cell |
|||
HTES1007 | Shanghai WSHT Biotechnology | set | EUR 259 |
myGel Mini Electrophoresis System 230V (includes transformer) |
|||
E1101-E | Benchmark Scientific | 1 PC | EUR 665.55 |
myGel Mini Electrophoresis System 230V (includes transformer) |
|||
BM0291 | GenDepot | System | EUR 789.6 |
CometAssay Electrophoresis Starter Kit plus EU Power Supply |
|||
4250-050-ESK-PS2 | Biotechne | 1 Kit | EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus UK Power Supply |
|||
4250-050-ESK-PS3 | Biotechne | 1 Kit | EUR 4597.68 |
CometAssay Electrophoresis Starter Kit plus SZ Power Supply |
|||
4250-050-ESK-PS4 | Biotechne | 1 Kit | EUR 4597.68 |
runVIEW horizontal electrophoresis system 15 x 7cm gel tray 1 set of casting dams 8 double sided combs - EACH |
|||
ELE2300 | Scientific Laboratory Supplies | EACH | EUR 1802.25 |
WSHT-midiDNA Multipurpose Horizontal Electrophoresis Cell |
|||
HTES1006 | Shanghai WSHT Biotechnology | set | EUR 278.6 |
SUPER-Green-I-nucleic-acid-gel-stain-*10,000×-concentrate-in-DMSO*-(Electrophoresis-Grade) |
|||
E37F001 | EnoGene | 100-μL | EUR 226.67 |
Description: Reagents-for-dsDNA-electrophoresis |
runVIEW horizontal electrophoresis system with 15 x 10cm gel tray 1 set of casting dams and 8 double sided combs - EACH |
|||
ELE2302 | Scientific Laboratory Supplies | EACH | EUR 1891.35 |
Agar, low gel strength |
|||
GK2227-1 | Glentham Life Sciences | 1 | EUR 110.7 |
Agar, low gel strength |
|||
GK2227-100 | Glentham Life Sciences | 100 | EUR 19.8 |
Agar, low gel strength |
|||
GK2227-100G | Glentham Life Sciences | 100 g | EUR 60 |
Agar, low gel strength |
|||
GK2227-1KG | Glentham Life Sciences | 1 kg | EUR 170.4 |
Agar, low gel strength |
|||
GK2227-250 | Glentham Life Sciences | 250 | EUR 35.5 |
Agar, low gel strength |
|||
GK2227-250G | Glentham Life Sciences | 250 g | EUR 79.2 |
Agar, low gel strength |
|||
GK2227-500 | Glentham Life Sciences | 500 | EUR 63.3 |
Agar, low gel strength |
|||
GK2227-500G | Glentham Life Sciences | 500 g | EUR 112.8 |
myGel InstaView™ Complete Electrophoresis System with Blue LED Illuminator |
|||
E1201 | Benchmark Scientific | 1 each | EUR 1001.7 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101, A1701 and W4000-100) |
|||
E1101-SK | Benchmark Scientific | 1 each | EUR 727.06 |
myGel Mini Electrophoresis System Starter Kit (Includes E1101-E, A1701 and W4000-100) |
|||
E1101-SK-E | Benchmark Scientific | 1 PC | EUR 757.35 |
In contrast, protein molecular weight ladder sizes ranging from 10 kDa to 225 kDa, built of blocks of the same polypeptide without post-translational modifications and others, showed activation of predicted energy requirements. Electrophoretic mobility of SDS – protein complex found root function-6 reciprocal of the molecular weight (Mw-sixth), implying a cylindrical conformation. Modifications give us a better result in terms of yield protein, resolution, spot number, and intensity for 2-DE gel of D. hirsuta and two other liverworts, namely, marchantia paleacea and Plagiochasma appendiculatum. In addition, we selected at random points on 2-DE gels D. hirsuta and identified using mass spectrometry, which confirmed the application of this protocol to liverworts proteomics.